PXD042538

PXD042538 is an original dataset announced via ProteomeXchange.

Title	Eukaryotic Initiation factor 4B is a multi-functional RNA binding protein that regulates histone mRNAs
Description	In diffuse large B-cell lymphoma (DLBCL) poor outcome is associated with EIF4B-dependent stimulation of the DEAD box helicase eIF4A that unwinds RNA structures in 5’ untranslated regions (UTRs) in mRNAs that encode proteins with a role in cell proliferation. We carried out iCLIP to examine differences in RNAs bound by eIF4B in DLBCL-derived- versus control B-cells and show that eIF4B bound ~10-fold more mRNAs in DLBCL-derived cells. In addition to interacting with 5' UTRs we unexpectedly find that eIF4B binds across the entire mRNA transcripts, with mutually exclusive, position-dependent binding related to different functional protein groups. Interestingly, there was an enrichment for binding within the 3’ UTR of mRNAs encoding replication-dependent histones, and consistent with this, we find that eIF4B interacts with UPF1, a helicase that plays a key role in the turnover of histone mRNAs at the end of S-phase. We identify a direct role for eIF4B in the turnover of histone mRNAs and demonstrate that eIF4B contributes to regulated cell cycle progression through controlling the translation and turnover of selected mRNAs.
HostingRepository	PRIDE
AnnounceDate	2024-09-04
AnnouncementXML	Submission_2024-09-04_05:22:31.669.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Harvey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-30 04:01:17	ID requested
⏵ 1	2024-09-04 05:22:32	announced

10.1093/NAR/GKAE767;

submitter keyword: eukaryotic initiation factor 4B (eIF4B)

mRNA translational control

histone mRNAs

RNA turnover

Anne Willis
contact affiliation	MRC Toxicology Unit, University of Cambridge, UK
contact email	aew80@mrc-tox.cam.ac.uk
lab head
Robert Harvey
contact affiliation	MRC Toxicology Unit, Universtiy of Cambridge
contact email	rfh32@mrc-tox.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD042538

PRIDE project URI

• PRIDE
  1. PXD042538
     1. Label: PRIDE project
     2. Name: Eukaryotic Initiation factor 4B is a multi-functional RNA binding protein that regulates histone mRNAs