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PXD042538

PXD042538 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEukaryotic Initiation factor 4B is a multi-functional RNA binding protein that regulates histone mRNAs
DescriptionIn diffuse large B-cell lymphoma (DLBCL) poor outcome is associated with EIF4B-dependent stimulation of the DEAD box helicase eIF4A that unwinds RNA structures in 5’ untranslated regions (UTRs) in mRNAs that encode proteins with a role in cell proliferation. We carried out iCLIP to examine differences in RNAs bound by eIF4B in DLBCL-derived- versus control B-cells and show that eIF4B bound ~10-fold more mRNAs in DLBCL-derived cells. In addition to interacting with 5' UTRs we unexpectedly find that eIF4B binds across the entire mRNA transcripts, with mutually exclusive, position-dependent binding related to different functional protein groups. Interestingly, there was an enrichment for binding within the 3’ UTR of mRNAs encoding replication-dependent histones, and consistent with this, we find that eIF4B interacts with UPF1, a helicase that plays a key role in the turnover of histone mRNAs at the end of S-phase. We identify a direct role for eIF4B in the turnover of histone mRNAs and demonstrate that eIF4B contributes to regulated cell cycle progression through controlling the translation and turnover of selected mRNAs.
HostingRepositoryPRIDE
AnnounceDate2024-09-04
AnnouncementXMLSubmission_2024-09-04_05:22:31.669.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRobert Harvey
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-30 04:01:17ID requested
12024-09-04 05:22:32announced
Publication List
10.1093/NAR/GKAE767;
Keyword List
submitter keyword: eukaryotic initiation factor 4B (eIF4B)
mRNA translational control
histone mRNAs
RNA turnover
Contact List
Anne Willis
contact affiliationMRC Toxicology Unit, University of Cambridge, UK
contact emailaew80@mrc-tox.cam.ac.uk
lab head
Robert Harvey
contact affiliationMRC Toxicology Unit, Universtiy of Cambridge
contact emailrfh32@mrc-tox.cam.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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