PXD042535

PXD042535 is an original dataset announced via ProteomeXchange.

Title	Comparing different pregnant mare serum gonadotropin products: surprising differences in protein content and a new sialic acid O-acetylation
Description	Equine chorionic gonadotropin (eCG) is a widely used hormone to synchronize the female cycle and induce estrusestruses in livestock breeding. eCG is a heterodimeric glycoprotein composed of non-covalently linked α- and β-chains whose glycosylation profiles determine the in vivo activity of the hormone. The commercially available eCG products are crudely purified from the serum of pregnant mares, hence called pregnant mare serum gonadotropin (PMSG). A correct glycosylation of the protein is crucial for the correct binding of the receptor, the half-life of the hormone and the intended function as such. The exact protein composition of the various commercial PMSG products and their specific glycosylation pattern have not been characterized so far. Therefore, in this study, we used proteomic approaches to analyze and compare four PMSG products. Here we show that the examined PMSGs share a surprisingly low level of commonalities (5.5%) in protein composition among the four tested products, with serum proteins as the main variable. Analysing the site-specific N-glycosylation, we identified for the first time the presence of O-acetylation of sialic acid at the structure of the glycan of eCG, which we could not find in significant amounts on human CG suggesting that this modification is species-specific.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:36:52.965.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042535
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chia-wei Lin
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-30 03:50:04	ID requested
⏵ 1	2025-05-06 09:36:53	announced

10.6019/PXD042535;

10.1038/s41598-025-90833-3;

L, ö, sle M, Lin CW, Beil-Wagner J, Aebi M, Buch T, Comparison of pregnant mare serum gonadotropin products with surprising differences in protein content. Sci Rep, 15(1):6824(2025) [pubmed]

submitter keyword: O-acetylation,equine chorionic gonadotropin (eCG) and lutropin, pregnant mare serum gonadotropin (PMSG), sulfation

Prof. Dr. Thorsten Buch
contact affiliation	Laboratory Animal Science/University of Zurich
contact email	thorsten.buch@uzh.ch
lab head
Chia-wei Lin
contact affiliation	Functional genomics center Zurich
contact email	lin.chiawei@fgcz.ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042535
     1. Label: PRIDE project
     2. Name: Comparing different pregnant mare serum gonadotropin products: surprising differences in protein content and a new sialic acid O-acetylation