PXD042513
PXD042513 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Combined transcriptomics and proteomics analysis unveiled the impact of vitamin C in modulating specific mitochondrial protein abundance in the mouse liver |
| Description | : In the present study, we used the Gulo-/- female and male mice model which depends entirely on ascorbate derived from the diet. We tested different concentrations of ascorbate ranging from 0 to 0.4% (w/v) ascorbate, added into drinking water, until the age of four months. Importantly, the levels of ascorbate found in the liver of Gulo-/- mice reflect the amounts of ascorbate provided in drinking water. We performed label-free Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) global quantitative proteomic profiling to identify and quantify proteins in whole liver extracts from our different ascorbate treated cohorts of Gulo-/- females and males. We compared the proteomic profiles to the transcriptomic profiles of the same treated animals. Although several proteins of the mitochondrial complex III significantly correlated with vitamin C concentrations, their corresponding transcripts did not correlate with vitamin C in both females and males. Such observations were confirmed by western blot and quantitative RT-PCR analyses, respectively. Our findings suggest that transcriptome profiling alone provides an incomplete picture of molecular changes associated with vitamin C deficiency in the liver of mice and examination of changes in protein abundances is essential to unveil variations that are not transcriptionally regulated. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-04-30 |
| AnnouncementXML | Submission_2024-04-30_07:53:10.532.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Michel Lebel |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | 4-hydroxy-L-proline |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-05-26 08:28:14 | ID requested | |
| ⏵ 1 | 2024-04-30 07:53:11 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Vitamin C, gulonolactone oxidase, mass spectrometry, mitochondrial complex III, transcription, liver, mouse |
Contact List
| Michel Lebel | |
|---|---|
| contact affiliation | Centre Hospitalier Universite Laval |
| contact email | michel.lebel@crchudequebec.ulaval.ca |
| lab head | |
| Michel Lebel | |
| contact affiliation | CHudeQuebe |
| contact email | michel.lebel@crchudequebec.ulaval.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000092040/ |
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