PXD042494

PXD042494 is an original dataset announced via ProteomeXchange.

Title	Comparison of LC-MS/MS and real-time PCR assays for the efficient detection of potentially allergenic silkworm
Description	The silkworm (Bombyx mori) has long been considered a source of food and medicine due to its high nutritional, medicinal, and economic value in East Asia. However, in some sensitive individuals, silkworm consumption can cause allergenic reactions such as vomiting, asthma, and anaphylaxis. Therefore, the development of a reliable method for silkworm detection is required to avoid such allergenic incidents. In this study, two different methods (liquid chromatography combined with mass spectrometry [LC-MS/MS] and real-time polymerase chain reaction [PCR]) were developed to determine an efficient technique for silkworm detection in foods. The developed methods demonstrated high sensitivity in detecting the silkworm in processed foods. Silkworm-spiked model cookies were used to confirm the sensitivity of both LC-MS/MS (0.0005%) and real-time PCR (0.001%). These methods were found to be useful for detecting the silkworm in foods and avoiding allergenic reactions. To the best of our knowledge, this is the first study to compare LC-MS/MS and real-time PCR for silkworm detection in complex processed foods.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_03:05:42.085.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kyungdo Kim
SpeciesList	scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-25 05:19:13	ID requested
⏵ 1	2024-05-24 03:05:42	announced

Suh SM, Kim K, Yang SM, Lee H, Jun M, Byun J, Lee H, Kim D, Lee D, Cha JE, Kim JS, Kim E, Park ZY, Kim HY, Comparative analysis of LC-MS/MS and real-time PCR assays for efficient detection of potential allergenic silkworm. Food Chem, 445():138761(2024) [pubmed]

10.1016/j.foodchem.2024.138761;

submitter keyword: Real-time PCR, Model cookies, Alpha-amlyase, Lipoprotein,Bombyx mori, Silkworm, LC-MS/MS

Zee-Yong Park
contact affiliation	School of Life Science, Gwangju Institute of Science and Technology, Cheomdangwagiro123, Buk-gu, Gwangju, 61005, Republic of Korea
contact email	zeeyong@gist.ac.kr
lab head
Kyungdo Kim
contact affiliation	Gwnagju Institute of Science and Technology
contact email	rlarudeh10@gm.gist.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD042494

PRIDE project URI

• PRIDE
  1. PXD042494
     1. Label: PRIDE project
     2. Name: Comparison of LC-MS/MS and real-time PCR assays for the efficient detection of potentially allergenic silkworm