PXD042470

PXD042470 is an original dataset announced via ProteomeXchange.

Title	The Centriolar Satellite Component CEP131 timely regulates mitochondrial Apoptosis
Description	Centriolar satellites are multiprotein aggregates that orbit the centrosome and govern centrosome homeostasis and primary cilia formation. In contrast to the scaffold PCM1, which nucleates centriolar satellites and has been linked to microtubule dynamics, autophagy, and intracellular trafficking, the functions of its close interactant CEP131 beyond ciliogenesis remain unclear. By using a knockout strategy in a T-cell line unable to form primary cilia, we report that, although dispensable for centriolar satellite assembly, CEP131 participates in optimal tubulin modifications and microtubule regrowth. Our unsupervised label-free proteomic analysis by quantitative mass spectrometry further uncovered both a mitochondrial and an anti-apoptotic signature. Without CEP131, mitochondria showed increased respiration and formed a more elongated network. In response to cell death inducers converging on mitochondria, knockout cells displayed delayed cytochrome c release from mitochondria, subsequent caspases activation, and ultimately in apoptosis. This defect in mitochondrial permeabilization was intrinsic as it could be recapitulated in vitro with isolated organelles. Together, these data extend the functions of CEP131 to life-and-death decisions and propose ways to interfere with mitochondrial apoptosis.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_10:40:08.745.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marjorie Leduc
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-24 03:35:00	ID requested
⏵ 1	2024-01-26 10:40:09	announced

Renaud CCN, Trillet K, Jardine J, Merlet L, Renoult O, Laurent-Blond M, Catinaud Z, Pecqueur C, Gavard J, Bid, è, re N, The centrosomal protein 131 participates in the regulation of mitochondrial apoptosis. Commun Biol, 6(1):1271(2023) [pubmed]

10.1038/s42003-023-05676-3;

submitter keyword: Mitochondria,Centriolar satellites,CEP131,Apoptosis

Nicolas BIDERE
contact affiliation	Team SOAP, CRCI2NA, Nantes University, INSERM, CNRS, Université d’Angers, Nantes, France / Equipe Labellisée Ligue Contre le Cancer, France
contact email	nicolas.bidere@inserm.fr
lab head
Marjorie Leduc
contact affiliation	Plateforme protéomique 3P5, Université de Paris, Institut Cochin, INSERM, CNRS,
contact email	marjorie.leduc@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD042470

PRIDE project URI

• PRIDE
  1. PXD042470
     1. Label: PRIDE project
     2. Name: The Centriolar Satellite Component CEP131 timely regulates mitochondrial Apoptosis