PXD042423

PXD042423 is an original dataset announced via ProteomeXchange.

Title	In-depth analysis of the pancreatic extracellular matrix during development for next generation tissue engineering
Description	The pancreas is a complex organ consisting of differentiated cells and extracellular matrix (ECM), which together enable its functions, including exocrine secretion and hormone production. Alt-hough much is known about the intrinsic factors that control pancreas development, under-standing of the microenvironment surrounding pancreatic cells remains incomplete. This envi-ronment is composed of various cells and ECM components, which play a critical role in main-taining tissue organization and homeostasis. In this study, we applied mass spectrometry to identify and quantify the ECM composition of the developing pancreas at embryonic E14.5 and perinatal P1 stages. Our proteomic analysis identified 160 ECM proteins that displayed a dynamic expression profile with a shift in collagens and proteoglycans. Furthermore, we used atomic force microscopy to measure the biomechanical properties of the ECM and found that the whole pan-creas ECM is relatively soft with no significant change during pancreas maturation. Finally, we developed a decellularization protocol for P1 pancreatic tissues, which preserved the 3D organi-zation of the ECM and can be used for recellularization studies. Our findings provide insights into the composition and biomechanics of the pancreatic embryonic and perinatal ECM, offering a foundation for future studies investigating the dynamic interactions between the ECM and pan-creatic cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:48:34.006.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042423
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Didier Vertommen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-23 04:21:07	ID requested
1	2023-06-19 02:27:26	announced
2	2023-11-14 06:44:26	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:48:34	announced	2024-10-22: Updated project metadata.

10.6019/PXD042423;

10.3390/IJMS241210268;

submitter keyword: Pancreas

extracellular matrix

development

decellularization

proteomics

atomic force mi-croscopy

Christophe E. Pierreux
contact affiliation	Cell Biology Unit, de Duve Institute, UCLouvain, 1200 Brussels, Belgium
contact email	christophe.pierreux@uclouvain.be
lab head
Didier Vertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD042423

PRIDE project URI

• PRIDE
  1. PXD042423
     1. Label: PRIDE project
     2. Name: In-depth analysis of the pancreatic extracellular matrix during development for next generation tissue engineering