PXD042413

PXD042413 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Absolute quantification of mTOR related signaling molecules
Description	To determine protein concentration in cells, we performed spike-in DIA experiments. Isotopically labeled concatemers (for mTOR-related signaling proteins) were spiked into lysates from p18Rev cells (each 20 mg) with four three-fold serial dilutions from 5,000 fmol. The lysates were subjected to digestion with trypsin. For absolute quantification of proteins, the digests containing isotopically labeled recombinant proteins were separated on a column (EV1106, Evosep) using a pre-programmed gradient (15 samples per day) at a flow rate of 200 nL/min using the Evosep One system (Evosep).
HostingRepository	jPOST
AnnounceDate	2024-05-23
AnnouncementXML	Submission_2024-05-22_08:00:19.762.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Masaki Matsumoto
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-22 23:22:21	ID requested
⏵ 1	2024-05-22 08:00:20	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: DIA, absolute quantification

submitter keyword: DIA, absolute quantification

Contact List

Masaki Matsumoto
lab head
Masaki Matsumoto
contact affiliation	Dep. Omics and Sys. Biol,, Niigata University Graduate School of Medical and Dental Sciences
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002164/