PXD042405

PXD042405 is an original dataset announced via ProteomeXchange.

Title	Insight into the dynamics of Ixodes ricinus nymphal midgut proteome
Description	The midgut of the tick is the most important tissue for the storage and digestion of host blood, which serves as the sole source of energy and nutrients for all tick development and reproduction. During feeding at each developmental stage, dynamic changes in the tick midgut epithelium reflect changes in physiological processes in this tissue. In addition, the midgut serves as the primary interface between the tick and tick-borne pathogens, which determines the vector competence of the tick. Several transcriptome data from Ixodes ricinus have been published, but few studies have examined tick proteomes. In contrast to transcriptome data, proteomics provides a deeper understanding of key cellular processes occurring in the investigated tissues. In this work, we present for the first time insight into proteomics of the midgut of I. ricinus nymph. Label-free quantitative proteomics was used to elucidate changes during blood meal and development in I. ricinus. A total of 1 534 I. ricinus-specific proteins were identified, with only a few host proteins detected. In addition, the proteins involved in the specific physiological processes of the tick were studied in detail to gain better insight into the ontogeny of the midgut of the nymph.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:54.139.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Filip Dyčka
SpeciesList	scientific name: Ixodes ricinus; NCBI TaxID: 34613;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-22 11:16:26	ID requested
1	2023-11-10 03:57:42	announced
2	2023-11-14 08:27:59	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:13:54	announced	2024-10-22: Updated project metadata.

10.1016/J.MCPRO.2023.100663;

submitter keyword: LC-MSMS, midgut, proteomics, tick,Ixodes ricinus, nymph

Petr Kopáček
contact affiliation	Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 1160/31, 37005 Ceske Budejovice, Czech Republic
contact email	kopajz@paru.cas.cz
lab head
Filip Dyčka
contact affiliation	University of South Bohemia in Ceske Budejovice Faculty of Science Branisovska 1760 Ceske Budejovice
contact email	dycka@mail.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD042405

PRIDE project URI

• PRIDE
  1. PXD042405
     1. Label: PRIDE project
     2. Name: Insight into the dynamics of Ixodes ricinus nymphal midgut proteome