PXD042402

PXD042402 is an original dataset announced via ProteomeXchange.

Title	Surface Proteomics of ADAM15 Knock-Down cells
Description	ADAM15 is a member of the disintegrin-metalloproteinase family of sheddases, which plays a role in several biological processes including cartilage homeostasis. Contrary to well-characterized ADAMs, little is known about ADAM15 substrates or how the enzyme exerts its biological functions. Herein, we used “surface-spanning enrichment with click-sugars (SUSPECS)” proteomics to identify ADAM15 substrates and/or proteins regulated by the proteinase at the surface of chondrocyte-like cells. ADAM15-silencing significantly altered membrane levels of 13 proteins, but none of these was a canonical substrate. We used orthogonal techniques to validate ADAM15 effects on 3 of these proteins which have known roles in cartilage homeostasis. This confirmed that ADAM15-silencing increased levels of PDCD1LG2 and reduced levels of vasorin and SLC26A2 through an unknown post-translational mechanism.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:20.829.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-22 08:45:37	ID requested
1	2023-06-13 06:29:38	announced
⏵ 2	2023-11-14 08:59:21	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: surface,ADAM15, SUSPECS

Simone Dario Scilabra
contact affiliation	Proteomics Group of Fondazione Ri.MED, Research Department IRCCS ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), via E. Tricomi 5, 90145 Palermo, Italy
contact email	sdscilabra@fondazionerimed.com
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD042402

PRIDE project URI

• PRIDE
  1. PXD042402
     1. Label: PRIDE project
     2. Name: Surface Proteomics of ADAM15 Knock-Down cells