PXD042394

PXD042394 is an original dataset announced via ProteomeXchange.

Title	Deconvoluting the complexity of Bacillus subtilis disulfide stress responses by redox-state and absolute abundance quantification of extracellular, membrane and cytosolic proteins
Description	To understand cellular mechanisms of stress management, omics data constitute a powerful resource. However, the availability of absolute quantitative data on protein abundances is still a serious limiting factor. This knowledge gap increments especially when protein abundances need to be compared across different cell compartments. In the present study, we aimed to provide deeper insights in the proteomic responses of the model Gram-positive bacterium Bacillus subtilis to disulfide stress. For this purpose, proteome-wide absolute abundances were determined with focus on different subcellular locations (cytosol and membrane) and the extracellular medium, and these were combined with redox state determination. To achieve the quantification of secreted proteins in the culture medium, a simple and straightforward protocol for the absolute quantification of extracellular proteins in bacteria was developed. Extracellular proteins, which are highly diluted in the medium, were concentrated using StrataClean beads together with a set of standard proteins that were used to determine the extent of the concentration step. The resulting dataset offers new insights into the protein abundances in different subcellular compartments and the extracellular medium, as well as a proteome-wide redox-state determination. Altogether, our study provides a quantitative understanding of disulfide stress management, protein production and secretion in B. subtilis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:28:36.817.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; TSQ Vantage

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-22 05:47:25	ID requested
1	2024-02-04 23:53:43	announced
⏵ 2	2024-10-22 06:28:37	announced	2024-10-22: Updated project metadata.

10.1128/SPECTRUM.02616-23;

submitter keyword: Absolute protein quantification / Bacillus subtilis / disulfide stress / redox proteomics / subcellular fractionation

Dörte Becher
contact affiliation	University of Greifswald, Institute of Microbiology, Department of Microbial Proteomics, Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042394
     1. Label: PRIDE project
     2. Name: Deconvoluting the complexity of Bacillus subtilis disulfide stress responses by redox-state and absolute abundance quantification of extracellular, membrane and cytosolic proteins