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PXD042390

PXD042390 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTesting the effect of histone native chemical ligation on protein binding to di-nucleosomes
DescriptionHere we examined whether histones H3 and H4 produced using native chemical ligation reaction affect protein binding to di-nucleosomes. To test this we performed a set of affinity purification pull-downs using di-nucleosomes containing the following histones H3 and H4: 1. unmodified ligated histone H3 and recombinant wild type histone H4 (unmod. H3), 2. recombinant wild type histone H3 and ligated unmodified histone H4 (unmod. H4) 3. ligated unmodified histones H3 and H4 (unmod. H3&H4). 4. recombinant wild type histones H3 and H4 (WT) Each pull-down was performed in three replicates. Co-purified proteins were quantified using label-free MS. Note that ligated histones H3 and H4 contain T32C or I29C single amino acid substitutions, respectively, that are introduced by the native chemical ligation procedure. In addition, ligated histone H4 is N-terminally acetylated to mimic its naturally blocked N-terminus.
HostingRepositoryPRIDE
AnnounceDate2024-04-29
AnnouncementXMLSubmission_2024-04-29_08:03:01.427.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndrey Tvardovskiy
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-22 03:17:59ID requested
12023-12-12 16:03:53announced
22024-04-29 08:03:02announced2024-04-29: Updated project metadata.
Publication List
Lukauskas S, Tvardovskiy A, Nguyen NV, Stadler M, Faull P, Ravnsborg T, Ö, zdemir Aygenli B, Dornauer S, Flynn H, Lindeboom RGH, Barth TK, Brockers K, Hauck SM, Vermeulen M, Snijders AP, M, ü, ller CL, DiMaggio PA, Jensen ON, Schneider R, Bartke T, Decoding chromatin states by proteomic profiling of nucleosome readers. Nature, 627(8004):671-679(2024) [pubmed]
10.1038/s41586-024-07141-5;
Keyword List
submitter keyword: nucleosome pull-down,native chemical ligation
Contact List
Till Bartke
contact affiliationInstitute of Functional Epigenetics, Helmholtz Zentrum München, 85764 Neuherberg, Germany.
contact emailtill.bartke@helmholtz-muenchen.de
lab head
Andrey Tvardovskiy
contact affiliationInstitute of Functional Epigenetics, Helmholtz Zentrum München, 85764 Neuherberg, Germany
contact emailtvardovsky.a@gmail.com
dataset submitter
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Dataset FTP location
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