Alkaliphilic microorganisms are similar to mesophilic counterparts with exception to membrane proteins. Detection of membrane proteins is difficult due to hydrophobicity and low absolute abundance. Caldalkalibacillus thermarum TA2.A1 is of interest due to growth from pH 7.5 to pH 10. Combination of whole cell lysate proteomics and membrane extracts solubilized with either SDS or FOS-choline-12 detected 158 membrane proteins, including a complete electron transport chain. Additionally, transporters for osmolytes ectoine and glycine betaine were observed.