PXD042364

PXD042364 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of FBP1 binding proteins in wild-type, G164D and F194S comparisons by LC-MS/MS analysis
Description	Myc-tagged FBP1-WT, Myc-tagged FBP1-G164D, Myc-tagged FBP1-F194S or empty vector were transfected into FBP1-KO HepG2 cells. FBP1 complexes were immunoprecipitated with anti-Myc beads and then separated by SDS‒PAGE. Bands were removed from the gel, and trypsin-digested samples were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an Orbitrap Velos Pro (Thermo Scientific). MS/MS dataset analysis was performed using the Mascot software program (Matrix Science).
HostingRepository	jPOST
AnnounceDate	2023-05-20
AnnouncementXML	Submission_2023-05-19_23:11:02.620.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yusuke Kawashima
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ Orbitrap Velos Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-19 22:56:11	ID requested
⏵ 1	2023-05-19 23:11:02	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: fructose 1,6-bisphosphatase deficiency, FBP1, hypoglycemia, protein misfolding, heat shock protein, HSP70, HSP90, HSP60, endoplasmic reticulum-associated degradation, ERAD

submitter keyword: fructose 1,6-bisphosphatase deficiency, FBP1, hypoglycemia, protein misfolding, heat shock protein, HSP70, HSP90, HSP60, endoplasmic reticulum-associated degradation, ERAD

Contact List

Tomoaki Tanaka
lab head
Yusuke Kawashima
contact affiliation	Kazusa DNA Research Institute
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002163/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002163/