PXD042353

PXD042353 is an original dataset announced via ProteomeXchange.

Title	The sex-specific factor SOA establishes X chromosome dosage compensation in Anopheles mosquitos
Description	The Anopheles mosquito is one of thousands of species in which sex differences play a central role in their biology, as only females need a blood meal in order to produce eggs. Sex differentiation is regulated by sex chromosomes, but their presence creates a dosage imbalance between males (XY) and females (XX). Dosage compensation (DC) can re-equilibrate the expression of sex-chromosomal genes, but because the molecular mechanisms providing DC have only been studied in a few model organisms, key questions about its evolutionary diversity and functional necessity remain unresolved. Here, we reveal the DC pathway in the malaria mosquito Anopheles gambiae. We identified SOA, a previously uncharacterized gene, whose expression is sufficient to induce a global upregulation of X-linked genes. Sex-specific alternative splicing prevents the production of a functional SOA protein in females. The male SOA isoform encodes a DNA-binding protein that recognizes the promoters of X chromosomal genes through a CA repeat sequence. Male mosquitos lacking SOA exhibit a chromosome-wide downregulation of the X, which is compatible with viability, but causes a developmental delay. Thus, our molecular analysis of the first DC master regulator in a non-model organism elucidates the evolutionary steps leading to the establishment of a chromosome-specific fine-tuning mechanism.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:53:53.798.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Anopheles gambiae (African malaria mosquito); NCBI TaxID: 7165;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-18 23:21:47	ID requested
1	2023-07-13 07:53:31	announced
2	2023-11-13 23:46:09	announced	2023-11-14: Updated project metadata.
3	2023-11-14 09:13:42	announced	2023-11-14: Updated project metadata.
⏵ 4	2024-10-22 05:53:54	announced	2024-10-22: Updated project metadata.

Kalita AI, Marois E, Kozielska M, Weissing FJ, Jaouen E, M, ö, ckel MM, R, ü, hle F, Butter F, Basilicata MF, Keller Valsecchi CI, The sex-specific factor SOA controls dosage compensation in Anopheles mosquitoes. Nature, 623(7985):175-182(2023) [pubmed]

10.1038/s41586-023-06641-0;

submitter keyword: LC-MS/MS

Falk Buttter
contact affiliation	Ackermannweg 4, 55128 Mainz, Germany
contact email	f.butter@imb.de
lab head
F Butter
contact affiliation	Quantitative Proteomics Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD042353

PRIDE project URI

• PRIDE
  1. PXD042353
     1. Label: PRIDE project
     2. Name: The sex-specific factor SOA establishes X chromosome dosage compensation in Anopheles mosquitos