PXD042348

PXD042348 is an original dataset announced via ProteomeXchange.

Title	Decoding the effects of drought stress on popcorn (Zea mays var. everta) flowering combining proteomics and physiological analysis
Description	Under soil water limitation and with adequate irrigation, we estimated growth measurements of the shoot and root system, as well as photosynthetic and proteomic profiles of two inbred lines of popcorn previously selected for tolerance to soil water limitation - L71, drought-tolerant; and L61, drought-sensitive - in an attempt to identify mechanisms related to tolerance to soil water limitation during the maturation phase. The plants were grown until grain filling in a substrate composed of perlite and peat in a 150 cm long PVC tube, under two water conditions (WC): i) irrigated (WW), at lysimeter capacity (LC - 100%); and ii) water-stressed (WS). The plants in the WS condition gradually reached 45% of the LC and were maintained like this for 10 days. Regardless of WC, L71 had the highest values of dry biomass of the shoot and root system, characterizing it as the most robust genotype. The imposed water limitation induced earlier senescence, degrading chlorophylls, and increasing anthocyanin values, with a more expressive impact in L61. The traits related to gas exchange expressed differences between lines only in the WS condition. In comparing WCs and most of the traits evaluated, the L71 genotype had the smallest proportional reductions. A total of 1,838 proteins were identified, with 169 differentially accumulated proteins (DAPs) in the tolerant line and 386 DAPs in the sensitive line. The pathways of energy metabolism, photosynthesis, oxidative stress response, and protein synthesis represented the main differences between L71 and L61. Therefore, our results provide important insights into the changes in proteomic profiles that occur in adaptation to water limitation in popcorn, providing useful information for breeding towards the expression of genotypic superiority to water deficit.
HostingRepository	PRIDE
AnnounceDate	2025-01-31
AnnouncementXML	Submission_2025-01-31_08:20:21.185.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vitor Pinto
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-18 17:28:43	ID requested
⏵ 1	2025-01-31 08:20:21	announced

10.1016/J.PLAPHY.2024.108444;

submitter keyword: abiotic stress, shotgun proteomics,Water deficit

Vitor Batista Pinto
contact affiliation	Laboratório de Biologia Celular e Tecidual Centro de Biociências e Biotecnologia Universidade Estadual do Norte Fluminense Darcy Ribeiro(UENF), Av. Alberto Lamego, Campos dos Goytacazes, RJ, Brazil.
contact email	vitorbp@uenf.br
lab head
Vitor Pinto
contact affiliation	Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)
contact email	vitorbp@uenf.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042348
     1. Label: PRIDE project
     2. Name: Decoding the effects of drought stress on popcorn (Zea mays var. everta) flowering combining proteomics and physiological analysis