PXD042346

PXD042346 is an original dataset announced via ProteomeXchange.

Title	The murine metastatic microenvironment of experimental brain metastases in breast cancer differs by host age in vivo: A proteomic study.
Description	Breast cancer in young patients is known to exhibit more aggressive biological behavior and is associated with a less favorable prognosis than the same disease in older patients, owing in part to an increased incidence of brain metastases, the mechanistic explanations behind which remain poorly understood. We recently reported that young mice, compared to older mice, showed about a three-fold increase in the development of brain metastases in mouse models of triple-negative and luminal B breast cancer. Here we have performed a quantitative mass spectrometry-based proteomic analysis to identify proteins contributing to age-related disparities in the development of breast cancer brain metastases. Using a mouse model of brain-tropic (MDA-MB-231BR) triple-negative breast cancer, we harvested subpopulations of tumor metastases, the tumor-adjacent metastatic microenvironment, and uninvolved brain tissues via laser microdissection followed by quantitative proteomic analysis using high resolution mass spectrometry to characterize differentially abundant proteins contributing to age-dependent rates of brain metastasis.
HostingRepository	PRIDE
AnnounceDate	2024-01-11
AnnouncementXML	Submission_2024-01-11_12:04:42.952.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Conrads
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-18 15:03:01	ID requested
⏵ 1	2024-01-11 12:04:43	announced

10.1007/S10585-023-10233-7;

submitter keyword: laser microdissection, mouse model, brain metastases,Breast cancer

Thomas P. Conrads
contact affiliation	Women’s Service Line Ionva Health System
contact email	conrads@whirc.org
lab head
Thomas Conrads
contact affiliation	Inova Health System
contact email	conrads@whirc.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042346
     1. Label: PRIDE project
     2. Name: The murine metastatic microenvironment of experimental brain metastases in breast cancer differs by host age in vivo: A proteomic study.