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PXD042342

PXD042342 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFibroblast-derived matrix models desmoplastic properties and forms a prognostic signature in cancer progression
DescriptionThe desmoplastic reaction observed in many cancers is a hallmark of disease progression and prognosis, particularly in breast and pancreatic cancer. Stromal-derived extracellular matrix (ECM) is significantly altered in desmoplasia, and as such plays a critical role in driving cancer progression. Using fibroblast-derived matrices (FDMs), we show that cancer cells have increased growth on cancer-associated FDMs, comparing to FDMs derived from non-malignant tissue (normal) fibroblasts. We assess the changes in ECM characteristics from normal to cancer-associated stroma at the primary tumor site. Compositional, structural, and mechanical analyses reveal significant differences, with an increase in abundance of core ECM proteins, coupled with an increase in stiffness and density in cancer-associated FDMs. From compositional changes of FDM we derived a 36-ECM proteins’ signature, which we show largely matching the changes in pancreatic ductal adenocarcinoma (PDAC) tumor and metastases progression, as well as at the transcriptomic level in multiple cancer types in patients, predictive of their survival. Together, our results show relevance of FDMs for cancer modelling and identification of desmoplastic ECM components for further mechanistic studies.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:54:06.516.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterErwin Schoof
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-18 12:53:36ID requested
12023-07-17 08:27:46announced
22023-08-08 00:53:13announced2023-08-08: Updated project metadata.
32023-08-08 01:21:41announced2023-08-08: Updated project metadata.
42023-11-14 09:04:42announced2023-11-14: Updated project metadata.
52024-10-22 05:54:07announced2024-10-22: Updated project metadata.
Publication List
Penner I, Dejung M, Freiwald A, Butter F, Chen JX, Plachter B, Proteome changes of fibroblasts and endothelial cells upon incubation with human cytomegalovirus subviral Dense Bodies. Sci Data, 10(1):517(2023) [pubmed]
10.1038/s41597-023-02418-2;
Keyword List
submitter keyword: cancer,lc-ms, matrix, mouse
Contact List
Janine Erler
contact affiliationBiotech Research and Innovation Centre (BRIC), University of Copenhagen (UCPH), Copenhagen, Denmark
contact emailjanine.erler@bric.ku.dk
lab head
Erwin Schoof
contact affiliationTechnical University of Denmark
contact emailerws@dtu.dk
dataset submitter
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Dataset FTP location
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