PXD042336

PXD042336 is an original dataset announced via ProteomeXchange.

Title	A novel strategy to characterize the pattern of β-lactam antibiotic-induced drug resistance in Acinetobacter baumannii.
Description	Carbapenem-resistant Acinetobacter baumannii (CRAb) is an urgent public health threat, according to the CDC. This pathogen has few treatment options and causes severe nosocomial infections with >50% fatality rate. Although previous studies have examined the proteome of CRAb, there have been no focused analyses of dynamic changes to β-lactamase expression that may occur due to drug exposure. Here, we present our initial proteomic study of variation in β-lactamase expression that occurs in CRAb with different β-lactam antibiotics. Briefly, drug resistance to Ab (ATCC 19606) was induced by the administration of various classes of β-lactam antibiotics, and the cell-free supernatant was isolated, concentrated, separated by SDS-PAGE, digested with trypsin, and identified by label-free LC-MS-based quantitative proteomics. Thirteen proteins were identified and evaluated using a 1789 sequence database of Ab β-lactamases from UniProt, the majority of which were Class C β-lactamases (≥80%). Importantly, different antibiotics, even those of the same class (e.g. penicillin and amoxicillin), induced non-equivalent responses comprising various isoforms of Class C and D serine-β-lactamases, resulting in unique resistomes. These results open the door to a new approach of analyzing and studying the problem of multi-drug resistance in bacteria that rely strongly on β-lactamase expression.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:30.634.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Belinda Willard
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Acinetobacter baumannii A424; NCBI TaxID: 1082934;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-18 10:29:23	ID requested
1	2023-07-20 11:45:43	announced
⏵ 2	2023-11-14 09:03:32	announced	2023-11-14: Updated project metadata.

Hillyer T, Benin BM, Sun C, Aguirre N, Willard B, Sham YY, Shin WS, -lactam antibiotic-induced drug resistance in Acinetobacter baumannii. Sci Rep, 13(1):9177(2023) [pubmed]

submitter keyword: tims Tof Pro2,Acinetobacter baumannii, DDA LFQ

Woo Shik Shin
contact affiliation	Department of Pharmaceutical Sciences, Northeast Ohio Medical University, Rootstown, OH
contact email	wshin@neomed.edu
lab head
Belinda Willard
contact affiliation	Cleveland Clinic
contact email	willarb@ccf.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042336
     1. Label: PRIDE project
     2. Name: A novel strategy to characterize the pattern of β-lactam antibiotic-induced drug resistance in Acinetobacter baumannii.