PXD042332

PXD042332 is an original dataset announced via ProteomeXchange.

Title	Evaluation of the impacts of detergent introduction on the target identification performance of TPP
Description	In this study, we assessed the target identification performance of TPP in the presence of a commonly used non-ionic detergent or a zwitterionic detergent using a pan-kinase inhibitor staurosporine, our results showed that the addition of either of these detergents significantly impaired the identification performance of TPP at the optimal temperature for soluble target protein identification. Further investigation showed that detergents destabilized the proteome and increased protein precipitation. By lowering the applied temperature point, target identifi-cation performance with detergents is significantly improved and is comparable to that in the absence of detergents. Our findings provide valuable insight into how to select the appropriate temperature range when detergents are used in TPP. In addition, our results also suggest that the combination of detergent and heat may serve as a novel precipitation-inducing force that can be applied for target protein identification.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:04:00.066.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yuying ye
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-18 09:33:19	ID requested
1	2023-07-20 12:41:28	announced
⏵ 2	2023-11-14 09:04:08	announced	2023-11-14: Updated project metadata.

Ye Y, Li K, Ma Y, Zhang X, Li Y, Yu T, Wang Y, Ye M, The Introduction of Detergents in Thermal Proteome Profiling Requires Lowering the Applied Temperatures for Efficient Target Protein Identification. Molecules, 28(12):(2023) [pubmed]

submitter keyword: mild detergents

thermal proteome profiling

target protein identification

Mingling Ye
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS)
contact email	mingliang@dicp.ac.cn
lab head
yuying ye
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS)
contact email	yuyingye1809@dicp.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042332
     1. Label: PRIDE project
     2. Name: Evaluation of the impacts of detergent introduction on the target identification performance of TPP