PXD042330

PXD042330 is an original dataset announced via ProteomeXchange.

Title	Pathogen protein modularity enables elaborate mimicry of a host phosphatase
Description	Pathogens produce diverse effector proteins to manipulate host cellular processes. However, how functional diversity is generated in an effector repertoire is poorly understood. Many effectors in the devastating plant pathogen Phytophthora contain tandem repeats of the “(L)WY” motif, which are structurally conserved but variable in sequences. Here, we discovered a functional module formed by a specific (L)WY-LWY combination in multiple Phytophthora effectors, which efficiently recruit the Serine/Threonine protein phosphatase 2A (PP2A) core enzyme in plant hosts. Crystal structure of an effector-PP2A complex shows that the (L)WY-LWY module enables hijacking of the host PP2A core enzyme to form functional holoenzymes. While sharing the PP2A-interacting module at the amino terminus, these effectors possess divergent C-terminal LWY units and regulate distinct sets of phosphoproteins in the host. Our results highlight the appropriation of an essential host phosphatase through molecular mimicry by pathogens and diversification promoted by protein modularity in an effector repertoire.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:50:50.114.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FRank Menke
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-18 09:29:34	ID requested
1	2023-06-26 08:42:31	announced
2	2023-11-14 06:53:27	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:50:50	announced	2024-10-22: Updated project metadata.

10.1016/J.CELL.2023.05.049;

submitter keyword: Arabidopsis, phosphatase holoenzyme,Virulence mechanism, microbial pathogenesis, Phosphoproteome, host-pathogen arms race

FRank Menke
contact affiliation	Sainsbury Laboratory Norwich
contact email	frank.menke@tsl.ac.uk
lab head
FRank Menke
contact affiliation	the Sainsbury laboratory
contact email	Frank.Menke@tsl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042330
     1. Label: PRIDE project
     2. Name: Pathogen protein modularity enables elaborate mimicry of a host phosphatase