PXD042322
PXD042322 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Immunoisolation of the trypanosome decapping enzyme ALPH1 delivers a potential subcomplex |
Description | Removal of mRNA 5’ caps primes transcripts for degradation and is central for regulating gene expression in eukaryotes. The canonical decapping enzyme DCP2 is stringently controlled by assembly into a dynamic multi-protein complex together with the 5´-3´exoribonuclease Xrn1. Kinetoplastida lack DCP2 orthologues but instead rely on the ApaH-like phosphatase ALPH1 for decapping. The enzyme is composed of a catalytic domain flanked by C-terminal and N-terminal extensions. In our related deposition PXD038550 we analysed the ALPH1 interactome by BioID proximity labelling for the full length protein and truncated versions in order to assign domain specific interactions. We showed that Trypanosoma brucei ALPH1 acts in a complex composed of the trypanosome XRN1 ortholog XRNA and four proteins that are unique to Kinetoplastida. The interactome was validated by reverse experiments targeting T. brucei and T. cruzi XRNA by affinity capture and, additionally, the ALPH1 interacting CMGC-family kinase by BioID. Here we carried out affinity capture with T. brucei and T. cruzi ALPH1, which delivers a potential sub-complex missing the C-terminal interactor XRNA. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:48:12.794.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martin Zoltner |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-05-18 05:41:55 | ID requested | |
1 | 2023-06-14 06:14:18 | announced | |
2 | 2023-11-14 09:00:47 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 05:48:13 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1093/nar/gkad497; |
Kramer S, Karolak NK, Odenwald J, Gabiatti B, Casta, ñ, eda Londo, ñ, o PA, Zav, ř, elov, á A, Freire ER, Almeida KS, Braune S, Moreira C, Eder A, Goos C, Field M, Carrington M, Holetz F, G, ó, rna MW, Zoltner M, A unique mRNA decapping complex in trypanosomes. Nucleic Acids Res, 51(14):7520-7540(2023) [pubmed] |
Keyword List
submitter keyword: XRNA, mRNA decay, ApaH-like phosphatase, ALPH1,mRNA decapping, Trypanosoma, exoribonuclease |
Contact List
Martin Zoltner | |
---|---|
contact affiliation | Head of Drug Discovery and Evaluation Center for Research of Pathogenicity and Virulence of Parasites Charles University in Prague, BIOCEV Průmyslová 595 252 50 Vestec Czech Republic |
contact email | zoltnerm@natur.cuni.cz |
lab head | |
Martin Zoltner | |
contact affiliation | Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH |
contact email | m.zoltner@dundee.ac.uk |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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