PXD042314

PXD042314 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicle-associated proteomic changes from autism patients before and after metabolic stimulation with long-term L-serine treatment
Description	The oral medication of L-serine decreased clinical global impression-severity (CGI-S) and clinical global impression-improvement (CGI-I). Behavioral scores, including communication, daily living skills, socialization, and adaptive behavior composite standardized scores, were estimated by distribution based MCID (Minimal clinically important difference) and all scores showed a trend of improvement. Since L-serine naturally cannot bypass the blood-brain barrier, we assumed L-serine could alter systemic molecular profiles, including protein expression in immune cells. In this study, we conducted plasma extracellular vesicle (EV) proteomic analysis to gain insight about immune changes in L-serine treated ASD patients. In order to study proteomic changes of EVs in plasma after oral medication for the ASD patients, we extracted EVs from the ASD patient’s plasma. In a study of nine ASD patients, we used LC-MS/MS to analyze changes in the proteome profile of plasma EVs before and after oral L-serine treatment. In each analysis, EVs were isolated, treated with trypsin for LC-MS/MS analysis, and processed through the PD software and several R packages.
HostingRepository	PRIDE
AnnounceDate	2025-10-23
AnnouncementXML	Submission_2025-10-22_22:39:39.616.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jaemyung Jang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-18 01:17:29	ID requested
⏵ 1	2025-10-22 22:39:40	announced

Dataset with its publication pending

submitter keyword: autism spectrum disorder,plasma extracellular vesicles, L-serine

Youngshik Choe
contact affiliation	Korea Brain Research Institute
contact email	dallarae@gmail.com
lab head
Jaemyung Jang
contact affiliation	Korea Brain Research Institute
contact email	piloter2@kbri.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD042314

PRIDE project URI

• PRIDE
  1. PXD042314
     1. Label: PRIDE project
     2. Name: Extracellular vesicle-associated proteomic changes from autism patients before and after metabolic stimulation with long-term L-serine treatment