This study was aimed to understand the changes of proteins occurring in atrial tissue of atrial fibrillation (AF) rat model based on proteome sequencing.SD rats were used for AF model construction by chronic intermittent hypoxia (CIH) induction. The tissue pathological changes were observed by hematoxylin-eosin staining, masson staining, and immunohistochemistry analysis of the levels of fibrosis biomarkers. Then proteome sequencing was performed on atrial fibrosis samples to identify the differentially expressed proteins (DEPs) between CIH and control groups, following by functional analysis. The protein levels of several key proteins were validated by western blotting.