PXD042308

PXD042308 is an original dataset announced via ProteomeXchange.

Title	Large-scale, quantitative proteomics reveals proteins required for planarian regeneration
Description	Multi-omics studies are increasingly used to decipher the mechanisms underlying tissue regeneration. However, the use of proteomics for such studies has been limited due to the high cost and relatively low throughput and reproducibility. The genes required for planarian regeneration have primarily been identified based on changes in mRNA transcripts; previous studies have not addressed the possibility of protein upregulation prior to detectable increases in transcript abundance during the early stages of planarian regeneration. Here, we used in-depth high-throughput proteomics approaches to analyze the model planarian Schmidtea mediterranea during regeneration and discovered 13 proteins that are essential regulators of key regeneration steps. Two of these proteins, Troponin T and MRPL18, are key regulators of regeneration initiation and showed increases in protein abundance prior to upregulation at the mRNA level. Extensive comparisons between proteomic and transcriptomic datasets suggested the critical mechanisms beyond the transcriptional level, such as the translational and post-translational levels. In summary, this study revealed key regulators of regeneration in a model organism; furthermore, it established an adaptable spectral library and comprehensive proteomics datasets, enabling future in-depth and multi-omics analyses of planarian biology.
HostingRepository	iProX
AnnounceDate	2023-05-17
AnnouncementXML	Submission_2026-05-08_19:19:35.409.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kai Lei
SpeciesList	scientific name: Schmidtea mediterranea; NCBI TaxID: 79327;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X; Q Exactive; TSQ Altis

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-17 20:21:31	ID requested
⏵ 1	2026-05-08 19:19:36	announced

Dataset with its publication pending

submitter keyword: proteomics, regeneration, wnt signal, wound response, ribosome

Kai Lei
contact affiliation	Westlake University, School of Life Sciences
contact email	leikai@westlake.edu.cn
lab head
Kai Lei
contact affiliation	Westlake University, School of Life Sciences
contact email	lei_lab@westlake.edu.cn
dataset submitter

iProX dataset URI