PXD042305

PXD042305 is an original dataset announced via ProteomeXchange.

Title	Glutamine supplementation alleviated aortic atherosclerosis in mice model and in vitro
Description	This study aimed to clarify the role of glutamine in atherosclerosis and its participating mechanism. Forty C57BL/6J mice were divided into wild control, ApoE-/- control, glutamine+ApoE-/-control, ApoE-/- high fat diet (HFD) and glutamine+ ApoE-/- HFD groups. The degree of atherosclerosis; plasma biochemistry and protein profiles, and western blotting and untargeted metabolomics of aortic tissue were detected at 18 weeks. An in vitro study was also performed. Glutamine treatment significantly decreased the degree of aortic atherosclerosis compared with that in the ApoE-/- HFD group (p=0.03). O-GlcNAc, IL-1β, IL-1α, GLUT1 and PKM2 in the ApoE-/- HFD group were significantly higher than those in the ApoE-/- control group (p < 0.05). These differences were reversed by glutamine treatment (p < 0.05), and aggravated by OGT overexpression in the in vitro study (p < 0.05). Multiomics showed that the ApoE-/- HFD group had higher levels of oxidative stress regulatory molecules (GUAD, XDH, PSB1 and PSB4), proinflammatory regulatory molecules (myristic acid, HMGB3, vinculin, DNJC2 and SPRC) and stress granules regulatory molecules (caprin-1 and DEAR) (p < 0.05). These differences were reversed by glutamine treatment (p < 0.05). We conclude that glutamine supplementation might alleviate atherosclerosis through downregulation of O-GlcNAc, glycolysis, oxidative stress and proinflammatory pathway.
HostingRepository	iProX
AnnounceDate	2023-05-15
AnnouncementXML	Submission_2023-08-22_18:51:26.647.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinggang Xia
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-17 18:41:22	ID requested
⏵ 1	2023-08-22 18:51:27	announced

Dataset with its publication pending

submitter keyword: atherosclerosis, inflammation, glutamine, glycolysis, oxidative stress

Jinggang Xia
contact affiliation	Xuanwu Hospital Capital Medical University
contact email	xiajinggang@sina.cn
lab head
Jinggang Xia
contact affiliation	Xuanwu Hospital Capital Medical University
contact email	xiajinggang@sina.cn
dataset submitter

iProX dataset URI