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PXD042298

PXD042298 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCapillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of mouse brain integral membrane proteins
DescriptionMass spectrometry (MS)-based top-down characterization of integral membrane proteins (IMPs) is crucial for understanding their functions in biological processes. However, it is technically challenging due to their low solubility in typical MS-compatible buffers. In this work, for the first time, we developed an efficient capillary zone electrophoresis (CZE)-tandem MS (MS/MS) method for top-down proteomics (TDP) of IMPs enriched from mouse brains. Our technique employs a sample buffer containing 30% (v/v) formic acid and 60% (v/v) methanol for solubilizing IMPs and utilizes a separation buffer of 30% (v/v) acetic acid and 30% (v/v) methanol for maintaining the solubility of IMPs during CZE separation. Single-shot CZE-MS/MS identified 51 IMP proteoforms from the mouse brain sample. Coupling size exclusion chromatography (SEC) to CZE-MS/MS enabled the identification of 276 IMP proteoforms from the mouse brain sample and those proteoforms contained 1-4 transmembrane domains. This proof-of-concept work demonstrates the high potential of CZE-MS/MS for large-scale TDP of IMPs.
HostingRepositoryPRIDE
AnnounceDate2023-12-07
AnnouncementXMLSubmission_2023-12-07_13:49:57.043.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLiangliang Sun
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; myristoylated residue; acetylated residue; monohydroxylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-17 13:14:46ID requested
12023-12-07 13:49:57announced
Publication List
10.1021/acs.analchem.3c02346;
Wang Q, Xu T, Fang F, Wang Q, Lundquist P, Sun L, Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Top-Down Proteomics of Mouse Brain Integral Membrane Proteins. Anal Chem, 95(34):12590-12594(2023) [pubmed]
Keyword List
submitter keyword: Top-down proteomics
Integral membrane protein
Proteoform
Capillary zone electrophoresis-mass spectrometry
Mouse brain
Contact List
Liangliang Sun
contact affiliationDepartment of Chemistry, Michigan State University, East Lansing, MI 48824
contact emaillsun@chemistry.msu.edu
lab head
Liangliang Sun
contact affiliationMichigan State University
contact emaillsun@chemistry.msu.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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