PXD042298 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Capillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of mouse brain integral membrane proteins |
Description | Mass spectrometry (MS)-based top-down characterization of integral membrane proteins (IMPs) is crucial for understanding their functions in biological processes. However, it is technically challenging due to their low solubility in typical MS-compatible buffers. In this work, for the first time, we developed an efficient capillary zone electrophoresis (CZE)-tandem MS (MS/MS) method for top-down proteomics (TDP) of IMPs enriched from mouse brains. Our technique employs a sample buffer containing 30% (v/v) formic acid and 60% (v/v) methanol for solubilizing IMPs and utilizes a separation buffer of 30% (v/v) acetic acid and 30% (v/v) methanol for maintaining the solubility of IMPs during CZE separation. Single-shot CZE-MS/MS identified 51 IMP proteoforms from the mouse brain sample. Coupling size exclusion chromatography (SEC) to CZE-MS/MS enabled the identification of 276 IMP proteoforms from the mouse brain sample and those proteoforms contained 1-4 transmembrane domains. This proof-of-concept work demonstrates the high potential of CZE-MS/MS for large-scale TDP of IMPs. |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-07 |
AnnouncementXML | Submission_2023-12-07_13:49:57.043.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Liangliang Sun |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue; myristoylated residue; acetylated residue; monohydroxylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-17 13:14:46 | ID requested | |
⏵ 1 | 2023-12-07 13:49:57 | announced | |
Publication List
10.1021/acs.analchem.3c02346; |
Wang Q, Xu T, Fang F, Wang Q, Lundquist P, Sun L, Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Top-Down Proteomics of Mouse Brain Integral Membrane Proteins. Anal Chem, 95(34):12590-12594(2023) [pubmed] |
Keyword List
submitter keyword: Top-down proteomics |
Integral membrane protein |
Proteoform |
Capillary zone electrophoresis-mass spectrometry |
Mouse brain |
Contact List
Liangliang Sun |
contact affiliation | Department of Chemistry, Michigan State University, East Lansing, MI 48824 |
contact email | lsun@chemistry.msu.edu |
lab head | |
Liangliang Sun |
contact affiliation | Michigan State University |
contact email | lsun@chemistry.msu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042298
- Label: PRIDE project
- Name: Capillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of mouse brain integral membrane proteins