PXD042288

PXD042288 is an original dataset announced via ProteomeXchange.

Title	Subcellular fractionation of Drosophila S2 cells upon foldamer treatment
Description	The use of synthetic chemicals to selectively interfere with chromatin and the chromatin-bound proteome represents a great opportunity for pharmacological intervention. Recently, synthetic foldamers that mimic the charge surface of double-stranded DNA have been shown to interfere with selected protein-DNA interactions. However, to better understand their pharmacological potential and to improve their specificity and selectivity their effect on complex chromatin needs to be investigated. We therefore systematically studied the influence of these DNA-mimicking foldamers on the chromatin-bound bound proteome using an in vitro chromatin assembly extract. Our studies revealed an efficient interference with the chromatin-associated origin recognition complex that plays an important role in initiating eukaryotic chromatin replication. This interference is mediated by a strong direct interaction between the foldamers and the origin recognition complex and has an effect in tissue culture cells where the addition of foldamer led to an S-phase arrest. Foldamers that mimic double-stranded nucleic acids thus emerge as a powerful tool with designable features to alter chromatin assembly and selectively interfere with biological mechanisms.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:07:27.711.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-17 06:47:43	ID requested
1	2023-09-04 05:29:40	announced
⏵ 2	2023-11-14 07:07:28	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: DNA mimics,Drosophila S2 cells, L2-4, subcellular fractionation

Prof. Dr. Axel Imhof
contact affiliation	Ludwig-Maximilians-Universität München Protein Analysis Unit Molecular Biology Department Großhadernerstr. 9 82152 Planegg-Martinsried
contact email	imhof@lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD042288

PRIDE project URI

• PRIDE
  1. PXD042288
     1. Label: PRIDE project
     2. Name: Subcellular fractionation of Drosophila S2 cells upon foldamer treatment