PXD042275

PXD042275 is an original dataset announced via ProteomeXchange.

Title	The silk gland proteome of Stenopsyche angustata provides insights into the underwater silk secretion
Description	Caddisworms (Trichoptera) spin adhesive silks to construct a variety of underwater composite structures. Many studies have focused on the fibroin heavy chain of caddisworm silk and found that it contains heavy phosphorylation to maintain a stable secondary structure. Besides fibroins, recent studies have also identified some new silk proteins within caddisworm silk. To better understand the silk composition and its secretion process, this study reports the silk gland proteome of a retreat-building caddisworm, Stenopsyche angustata. Using LC-MS/MS, 2389 proteins were identified in the silk gland of S. angustata, among which 192 were predicted as secreted silk proteins. Twenty-nine proteins were found to be enriched in the front silk gland, while 109 proteins were enriched in the caudal silk gland. The fibroin heavy chain and nine uncharacterized silk proteins were identified as phosphorylated proteins. By analyzing the sequence of the fibroin heavy chain, we found it contains 13 Gly/Thr/Pro-rich regions, 12 Val/Ser/Arg-rich regions, and a Gly/Arg/Thr-rich region. Three uncharacterized proteins were identified as sericin-like proteins due to having larger molecular weights, signal peptides, and repetitive motifs rich in serine. This study provides valuable information for further clarifying the secretion and adhesion of underwater caddisworm silk.
HostingRepository	iProX
AnnounceDate	2023-05-16
AnnouncementXML	Submission_2023-05-16_18:42:08.110.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhaoming Dong
SpeciesList	scientific name: Stenopsyche angustata; NCBI TaxID: 1560148;
ModificationList	phosphorylated residue; N-acetylated residue; methionine oxidation with neutral loss of 64 Da
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-16 18:41:18	ID requested
⏵ 1	2023-05-16 18:42:08	announced

Dataset with its publication pending

submitter keyword: Silk gland, Fibroin, Phosphorylation, Caddisfly, Trichoptera

Qingyou Xia
contact affiliation	Southwest University
contact email	xiaqy@swu.edu.cn
lab head
Zhaoming Dong
contact affiliation	Southwest University
contact email	dongzhaoming@swu.edu.cn
dataset submitter

iProX dataset URI