PXD042270

PXD042270 is an original dataset announced via ProteomeXchange.

Title	Tectonic Complex Impedes Diffusion through the Ciliary Transition Zone to Ensure Proper Sorting of Membrane Proteins
Description	The primary cilium is a signaling organelle with a unique membrane composition maintained by a diffusional barrier residing at the transition zone. Many transition zone proteins, such as the tectonic complex, are linked to preserving ciliary composition; however, whether these proteins regulate active transport or physically impede membrane diffusion remains unknown. To understand tectonic’s role, we generated a photoreceptor specific Tctn1 knockout mouse. Loss of Tctn1 resulted in an absence of the entire tectonic complex yet had minimal effects on transition zone structure. Interestingly, we found that protein composition of the photoreceptor cilium was disrupted as non-resident membrane proteins accumulated in the cilium over time, ultimately resulting in photoreceptor degeneration. We further show that membrane proteins moved faster through the transition zone illustrating that the tectonic complex acts as a physical barrier to slow diffusion of membrane proteins so they can be properly sorted by ciliary transport carriers.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:10:10.744.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jillian Pearring
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-16 11:47:29	ID requested
1	2023-10-24 12:53:19	announced
2	2023-11-14 09:10:46	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:10:11	announced	2024-10-22: Updated project metadata.

10.1038/s41467-023-41450-z;

Truong HM, Cruz-Col, ó, n KO, Mart, í, nez-M, á, rquez JY, Willer JR, Travis AM, Biswas SK, Lo WK, Bolz HJ, Pearring JN, The tectonic complex regulates membrane protein composition in the photoreceptor cilium. Nat Commun, 14(1):5671(2023) [pubmed]

submitter keyword: cilia,Tectonic, photoreceptor

Jillian N. Pearring
contact affiliation	Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, MI, USA Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA
contact email	pearring@umich.edu
lab head
Jillian Pearring
contact affiliation	University of Michigan
contact email	pearring@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042270

PRIDE project URI

• PRIDE
  1. PXD042270
     1. Label: PRIDE project
     2. Name: Tectonic Complex Impedes Diffusion through the Ciliary Transition Zone to Ensure Proper Sorting of Membrane Proteins