PXD042238

PXD042238 is an original dataset announced via ProteomeXchange.

Title	ZC3H11A interacts with PABPN1 and alters polyadenylation of viral transcripts
Description	Nuclear mRNA metabolism is regulated by multiple proteins, which either directly bind to RNA or form multi-protein complexes. The RNA-binding protein ZC3H11A is involved in nuclear mRNA export, and NF-κB signaling and is essential during mouse embryo development. Furthermore, previous studies have shown that ZC3H11A is important for nuclear-replicating viruses. However, detailed biochemical characterization of the ZC3H11A protein has been lacking. In this study, we established the ZC3H11A protein interactome in human and mouse cells. We demonstrate that the nuclear poly(A)-binding protein PABPN1 interacts specifically with the ZC3H11A protein and controls ZC3H11A localization into nuclear speckles. We report that ZC3H11A specifically interacts with the human adenovirus type 5 (HAdV-5) capsid mRNA in a PABPN1-dependent manner. Notably, ZC3H11A uses the same zinc finger motifs to interact with PABPN1 and mRNA, with a single zinc finger motif responsible for these functions. Further, we demonstrate that the lack of ZC3H11A alters the polyadenylation of HAdV-5 capsid mRNA. Taken together, our results suggest that the ZC3H11A protein acts as a novel regulator of polyadenylation of nuclear mRNA.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:10:41.775.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mårten Larsson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-15 09:22:30	ID requested
1	2023-10-24 12:52:47	announced
⏵ 2	2023-11-14 09:10:49	announced	2023-11-14: Updated project metadata.

Kases K, Schubert E, Hajikhezri Z, Larsson M, Devi P, Darweesh M, Andersson L, Akusj, ä, rvi G, Punga T, Younis S, The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts. J Biol Chem, 299(8):104959(2023) [pubmed]

submitter keyword: zinc finger protein,PABPN1, ZC3H11A, adenovirus, polyadenylation

Tanel Punga
contact affiliation	Uppsala University, Uppsala, Sweden
contact email	Tanel.Punga@imbim.uu.se
lab head
Mårten Larsson
contact affiliation	Uppsala University
contact email	Marten.Larsson@imbim.uu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042238
     1. Label: PRIDE project
     2. Name: ZC3H11A interacts with PABPN1 and alters polyadenylation of viral transcripts