PXD042236

PXD042236 is an original dataset announced via ProteomeXchange.

Title	Monitoring SUMO-2 proteome changes during adenovirus infection using WT and an E1B-55k mutant.
Description	The human adenovirus type 5 (HAdV-C5) early region 1B 55-kDa protein (E1B-55K) is a multifunctional protein that promotes viral replication and adenovirus-mediated cell transformation through various mechanisms that primarily counteract host intrinsic and innate immunity. These include post-translational activities that exploit the host cell ubiquitin- and SUMO-conjugation machineries to regulate antiviral cellular restriction factors. However, despite significant advancements in this field, several underlying mechanisms governing these processes remain unidentified to date. Here, we performed SILAC-based quantitative SUMO proteomics to better understand cellular consequences of E1B-55K-mediated host cell modulation and adenovirus infection in general. We assessed cellular proteins for abundance changes and SUMO2 conjugate proteome changes during infection with wild type HAdV-C5 or E1B-55K deletion mutants. We provide evidence that changes in the SUMOylated proteome have the potential to regulate the DNA damage response, cell cycle control, chromatin assembly, and gene transcription. Strikingly, we identified a SUMO-dependent ubiquitin-mediated degradation mechanism for some SUMO substrates, suggesting that E1B-55K may use multiple mechanisms to alter the activity of restrictive cellular pathways.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:06:03.851.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mike Tatham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-15 07:14:39	ID requested
1	2023-10-17 10:16:20	announced
2	2023-11-14 07:47:28	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:06:04	announced	2024-10-22: Updated project metadata.

10.1128/JVI.00791-23;

submitter keyword: SUMO Adenovirus E1B-55K

Ronald Thomas
contact affiliation	Division of Molecular, Cell and Developmental Biology, School of Life Sciences, University of Dundee,
contact email	r.t.hay@dundee.ac.uk
lab head
Mike Tatham
contact affiliation	University of Dundee
contact email	m.tatham@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042236
     1. Label: PRIDE project
     2. Name: Monitoring SUMO-2 proteome changes during adenovirus infection using WT and an E1B-55k mutant.