PXD042226

PXD042226 is an original dataset announced via ProteomeXchange.

Title	Identify HILPS-interacting proteins and HIF1a phosphorylation sites
Description	To dissect the molecular mechanisms of HILPS in promoting cell survival under hypoxia, we conducted RNA pull-down assay in combination with mass spectrometry analysis to identify HILPS-interacting proteins. Pull-down of antisense HILPS was used as a negative control. To identify the potential phosphorylation site(s), we conducted the in vitro kinase assay in combination with mass spectrometry analysis using PLK1-T210D, recombinant GST-HIF1a and cold ATP. A kinase assay using PLK1-K82R was included as a control.
HostingRepository	PRIDE
AnnounceDate	2023-10-19
AnnouncementXML	Submission_2023-10-18_20:12:47.881.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ZhiChen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-15 03:05:09	ID requested
⏵ 1	2023-10-18 20:12:48	announced

Dataset with its publication pending

submitter keyword: human, LC-MS/MS

GuoliangQing
contact affiliation	Wuhan University, Wuhan, China
contact email	qingguoliang@whu.edu.cn
lab head
ZhiChen
contact affiliation	Wuhan University
contact email	chenzhi027@whu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042226

PRIDE project URI

• PRIDE
  1. PXD042226
     1. Label: PRIDE project
     2. Name: Identify HILPS-interacting proteins and HIF1a phosphorylation sites