PXD042222

PXD042222 is an original dataset announced via ProteomeXchange.

Title	TFIP11 competes with the Bloom syndrome helicase for binding to stalled replication forks to preserve genome stability
Description	Replication fork reversal is a key protective mechanism against replication stress in higher eukaryotic cells and occurs via a series of coordinated enzymatic reactions. The Bloom syndrome gene product, BLM, is a member of the highly conserved RecQ helicase family and has been implicated in this process, but its precise regulation and role remain poorly understood. Here, we show that, upon replication stress, the GCFC domain-containing protein TFIP11 competes with the BLM helicase for association with stalled replication forks, thereby facilitates RAD51-mediated stalled fork reversal. Consequently, loss of TFIP11 results in aberrant accumulation of BLM at stalled forks, which in turn compromises RAD51 recruitment, impairs replication stress-induced fork reversal and slowing, hypersensitizes cells to replication stress-inducing agents, and enhances chromosomal instability. These findings reveal a previously unidentified regulatory mechanism that modulates the activities of BLM and RAD51 at stalled forks and thus genome integrity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:33.031.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junliang Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-15 02:47:43	ID requested
1	2024-05-24 02:04:19	announced
⏵ 2	2024-10-22 06:42:34	announced	2024-10-22: Updated project metadata.

10.1038/s41467-024-45684-3;

Chen J, Wu M, Yang Y, Ruan C, Luo Y, Song L, Wu T, Huang J, Yang B, Liu T, TFIP11 promotes replication fork reversal to preserve genome stability. Nat Commun, 15(1):1262(2024) [pubmed]

submitter keyword: Bloom syndrome helicase

Replication stress

Replication fork reversal

Genome instability

TFIP11

Ting Liu
contact affiliation	Department of Cell Biology, Zhejiang University School of Medicine, Hangzhou, 310058, China
contact email	liuting518@zju.edu.cn
lab head
Junliang Chen
contact affiliation	15267493097
contact email	11707006@zju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD042222

PRIDE project URI

• PRIDE
  1. PXD042222
     1. Label: PRIDE project
     2. Name: TFIP11 competes with the Bloom syndrome helicase for binding to stalled replication forks to preserve genome stability