PXD042221 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes |
Description | Recombination-mediated linking of homologous chromosomes is a unique meiotic feature that enables the halving of chromosomal ploidy in sexual life cycles. Meiotic recombination is initiated by programmed DNA double-strand breaks (DSBs) that are generated by focal multi-protein clusters that condense onto chromosomal axes by poorly understood mechanisms. We discovered in mice that a DBF4-dependent kinase (DDK)–modulated interaction between IHO1 and the chromosomal axis component HORMAD1 effects the formation of axis-bound IHO1 platforms that enhance nucleation of cytologically distinguishable DSB-machinery clusters. This IHO1-HORMAD1-mediated seeding of the DSB-machinery ensures that sufficient DSBs form for efficient pairing of homologous chromosomes. In the absence of IHO1-HORMAD1 interaction, residual DSB activity depends on ANKRD31, which enhances both the seeding and the growth of DSB-machinery clusters. Thus, meiotic DSBs are ensured by complementing pathways that differentially effect seeding and growth of DSB-machinery clusters, and that synergistically enable assembly of the DSB machinery on chromosomal axes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:31:29.584.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD042221 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Benjamin Neuditschko |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-15 02:18:52 | ID requested | |
1 | 2024-02-23 02:39:38 | announced | |
⏵ 2 | 2024-10-22 06:31:30 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: IHO1, Phosphorylation |
Contact List
Franz Herzog |
contact affiliation | Institute Krems Bioanalytics, IMC University of Applied Sciences, 3500 Krems, Austria |
contact email | franz.herzog@fh-krems.ac.at |
lab head | |
Benjamin Neuditschko |
contact affiliation | Institute Krems Bioanalytics, IMC University of Applied Sciences Krems, 3500 Krems, Austria |
contact email | benjamin.neuditschko@fh-krems.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042221
- Label: PRIDE project
- Name: Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes