PXD042219

PXD042219 is an original dataset announced via ProteomeXchange.

Title	Development and systematic evaluation of decellularization protocols for rat diaphragms
Description	We decellularized rat diaphragms with the main detergents 1% or 0.1% sodium dodecyl sulfate (SDS) and 4% sodium deoxycholate (SDC) by orbital shaking (OS) or retrograde perfusion (RP) through the vena cava. Our systematic comparison entails evaluation of decellularized diaphragmatic samples by (1) quantitative analysis including DNA quantification and biomechanical testing, (2) qualitative and semi-quantitative analysis by proteomics, as well as (3) qualitative assessment with macroscopic and microscopic evaluation by histological staining, immunohistochemistry and scanning electron microscopy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:38:30.107.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karl Hillebrandt
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-15 01:48:18	ID requested
1	2024-05-07 11:45:30	announced
⏵ 2	2024-10-22 06:38:30	announced	2024-10-22: Updated project metadata.

Andreas MN, Boehm AK, Tang P, Moosburner S, Klein O, Daneshgar A, Ga, ß, ner JMGV, Raschzok N, Haderer L, Wulsten D, R, ü, ckert JC, Spuler S, Pratschke J, Sauer IM, Hillebrandt KH, Development and systematic evaluation of decellularization protocols in different application models for diaphragmatic tissue engineering. Biomater Adv, 153():213493(2023) [pubmed]

10.1016/j.bioadv.2023.213493;

submitter keyword: decellularized rat diaphragm proteomics

Karl Herbert Hillebrandt
contact affiliation	Department of Surgery, Charité Universitätsmedizin Berlin
contact email	karl-herbert.hillebrandt@charite.de
lab head
Karl Hillebrandt
contact affiliation	Department of Surgery
contact email	karl-herbert.hillebrandt@charite.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD042219

PRIDE project URI

• PRIDE
  1. PXD042219
     1. Label: PRIDE project
     2. Name: Development and systematic evaluation of decellularization protocols for rat diaphragms