PXD042218

PXD042218 is an original dataset announced via ProteomeXchange.

Title	PC4-mediated Ku PARylation facilitates NHEJ-dependent DNA damage repair
Description	Radiotherapy is one of the mainstays for the treatment of hepatocellular carcinoma (HCC); however, a substantial fraction of HCC patients develops radioresistance and eventually suffer from tumour progression or relapse, a major impediment to the use of radiotherapy. One of the main goals in HCC management is to elucidate the mechanisms underlying radioresistance and identify novel therapeutic targets to improve the patients' prognosis. In this study, we report a DNA repair enhancer, human positive cofactor 4 (PC4), that promotes NHEJ-based DNA repair and renders HCC cells resistant to radiation. Mechanistically, PC4 interacts with PARP1 and directs Ku complex PARylation, resulting in successful recruitment of the Ku complex to damaged chromatin and increasing the efficiency of NHEJ repair. Clinically, PC4 is highly expressed in tumour tissues, which is correlated with poor prognosis in HCC patients. Taken together, our data suggest that PC4 is a DNA repair driver that can be targeted to radiosensitize HCC.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:10:41.520.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qimei Pan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-15 01:37:46	ID requested
1	2023-10-24 12:46:16	announced
⏵ 2	2023-11-14 09:10:46	announced	2023-11-14: Updated project metadata.

Pan Q, Luo P, Shi C, PC4-mediated Ku complex PARylation facilitates NHEJ-dependent DNA damage repair. J Biol Chem, 299(8):105032(2023) [pubmed]

submitter keyword: PC4, MS, liver cancer

Chummeng Shi
contact affiliation	Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University)
contact email	shicm@tmmu.edu.cn
lab head
Qimei Pan
contact affiliation	Army Medical University
contact email	mayble_pan@sina.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042218

PRIDE project URI

• PRIDE
  1. PXD042218
     1. Label: PRIDE project
     2. Name: PC4-mediated Ku PARylation facilitates NHEJ-dependent DNA damage repair