PXD042217

PXD042217 is an original dataset announced via ProteomeXchange.

Title	A qualitative proteome-wide lysine crotonylation profiling reveals protein modification alteration in the leukocyte extravasation pathway in systemic lupus erythematosus
Description	Background Systemic lupus erythematosus (SLE) is a severe systemic autoimmune disease with multiple manifestations. Lysine crotonylation (Kcr) is a newly discovered post-translational modification (PTM) epigenetic pattern which may affect gene expression and linked to diseases causally. Methods We collected blood samples from 11 SLE individuals and 36 healthy subjects. Then we used highly sensitive liquid chromatography-mass spectrometry technology to carry out proteomics and quantitative crotonylome analysis of SLE peripheral blood mononuclear cells in this investigation, which indicated the unique etiology of SLE. Results There were 618 differentially expressed proteins (DEPs), and 612 crotonylated lysine sites for 272 differentially modified proteins (DMPs) found. According to KEGG analysis and ingenuity pathway analysis, these DEPs and DMPs are primarily enriched in the leukocyte extravasation signaling pathway. Conclusions This is the first study of crotonylated modification proteomics in SLE. The leukocyte extravasation signaling pathway had a considerable concentration of DEPs and DMPs, indicating that this pathway may be involved in the pathogenic development of SLE.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:26:47.132.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huiyi Zeng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	crotonylated L-lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-14 23:20:15	ID requested
1	2024-01-26 08:19:21	announced
⏵ 2	2024-10-22 06:26:48	announced	2024-10-22: Updated project metadata.

Zeng H, Li D, Dong J, Zhou X, Ou M, Xue W, Zhang R, Zou Y, Tang D, Yin L, Dai Y, Qualitative Proteome-wide Lysine Crotonylation Profiling Reveals Protein Modification Alteration in the Leukocyte Extravasation Pathway in Systemic Lupus Erythematosus. ACS Omega, 8(47):44905-44919(2023) [pubmed]

10.1021/acsomega.3c06293;

submitter keyword: Systemic lupus erythematosus

Proteome

Crotonylation

Post-translational modifications

Leukocyte extravasation

Yong Dai
contact affiliation	the Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen
contact email	daiyong22@aliyun.com
lab head
Huiyi Zeng
contact affiliation	Institute of Nephrology and Blood Purification, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong
contact email	2235538311@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042217
     1. Label: PRIDE project
     2. Name: A qualitative proteome-wide lysine crotonylation profiling reveals protein modification alteration in the leukocyte extravasation pathway in systemic lupus erythematosus