PXD042202

PXD042202 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Ribosome associated proteins of functional ribosomes from murine spermatocytes and round spermatids
Description	Murine spermatocytes and round spermatids were isolated by STA-PUT method (J Vis Exp. 2013; (80): 50648.). In order to avoid contamination of nuclear ribosomal subunits and proteins, cytoplasm fractions were extracted by REAP method(BMC Res Notes 3, 294 (2010).) right after cell collection. Immunoprecipitation of functional ribosomes was performed by targeting Rpl36 with the corresponding antibody (Bethyl Laboratories, A305065A). This procedure to capture functional ribosomes was supported by a previous publication (Cell . 2017 Jun 1;169(6):1051-1065.e18.). Rabbit IgG (Cell Signalling Technology, #2729) was used as the negative control. Quantitative mass spectrometry analyses were performed by TMT10plex Isobaric Label Reagents and Kits (Thermo Scientific, 90110).
HostingRepository	MassIVE
AnnounceDate	2023-10-15
AnnouncementXML	Submission_2023-10-15_02:42:52.242.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joulab
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-13 01:54:41	ID requested
⏵ 1	2023-10-15 02:42:52	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Ribosome, Rpl36, Spermatogenesis, Spermatocyte, Round spermatid

submitter keyword: Ribosome, Rpl36, Spermatogenesis, Spermatocyte, Round spermatid

Contact List

Yuh-Shan Jou
contact affiliation	Institute of Biomedical Science, Academia Sinica
contact email	jou@ibms.sinica.edu.tw
lab head
Joulab
contact affiliation	institute of biomedical science, Academia Sinica
contact email	userlee7655@ibms.sinica.edu.tw
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091932/