PXD042198
PXD042198 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Development of a deep proteomic pipeline for recalcitrant olive leaf tissue |
| Description | Proteomic analysis is a powerful tool to unravel the complexity of plant cellular processes that underpin the regulation of plant immunity. A major challenge is the improvement of the detectable fraction of the crop proteome that is still markedly lower compared to other omics, such as next generation sequencing technologies. This is due in part to the occurrence of large amounts of secondary compounds, which co-precipitate with proteins and severely interfere with the analysis. Olive leaf tissue is notoriously recalcitrant to common protein extraction methods due to high levels of interfering compounds, hence hampering deep proteomic investigations. The interest in the chemical composition of olive leaves has increased with the scope to re-evaluate this agricultural waste byproduct as their extracts are enriched in diverse bioactive compounds. Many of these secondary metabolites are involved in the defense systems along with their biosynthesis enzymes, whose activity is usually cultivar- and stimuli-dependent. Despite olive leaf proteomics providing important insights into the defense pathways as well as health diagnostic biomarkers, it has received much less attention compared to oil, drupes, seed and pollen tissues. Our study aims to overcome these hurdles and expand the application of deep proteomic analyses to olive leaves. We developed a complete proteomic pipeline, from sample preparation to LC-HRMS and data analyses, allowing the first comparative proteomic study among three Italian olive cultivars, i.e., Leccino, Ogliarola and Coratina, known to exhibit different susceptibility to Xylella fastidiosa infections, and enabling the detection of 1.922 proteins. Olive proteomic research is expected to become an essential part of integrated omics approaches; thus, our study is a significant contribution, paving the way to unravel the molecular complexity underlying the genotype-dependent immune response to stress. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-11-01 |
| AnnouncementXML | Submission_2023-11-01_11:11:39.873.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Brett Phinney |
| SpeciesList | scientific name: Xylella fastidiosa; NCBI TaxID: 2371; scientific name: Olea europaea; common name: common olive; NCBI TaxID: 4146; |
| ModificationList | Oxidation |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-05-12 16:59:38 | ID requested | |
| ⏵ 1 | 2023-11-01 11:11:40 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: olive leaf tissue, Xylella fastidiosa , Crop proteomics, Olive proteomics, olive leaf proteomics |
Contact List
| Abhaya Dandekar | |
|---|---|
| contact affiliation | University of California, Davis |
| contact email | amdandekar@ucdavis.edu |
| lab head | |
| Brett Phinney | |
| contact affiliation | UC Davis |
| contact email | brettsp@ucdavis.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091931/ |
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