PXD042182

PXD042182 is an original dataset announced via ProteomeXchange.

Title	Multi-omics analysis of mutagenized S. rimosus reveals synergistic changes that drive oxytetracycline production towards industrial efficiency and opens a new route to heterologous polyketides
Description	The present work provides a multi-omics systems-wide view on S. rimosus. Using genomics, transcriptomics, proteomics, and metabolomics, we compared the wild type with an OTC-overproducing derivative, previously obtained by classical mutagenesis. The integration of the data provided a deep insight into the underlying metabolic and regulatory networks that mediate high-level OTC formation. Strikingly, the overproducer revealed a synergistically activated supply of acetyl-CoA and malonyl CoA and increased abundance of various CoA thioesters.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_08:49:00.609.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Rückert-Reed
SpeciesList	scientific name: Streptomyces rimosus subsp. rimosus; NCBI TaxID: 132474;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-12 06:46:30	ID requested
⏵ 1	2024-01-26 08:49:01	announced

Beganovic S, R, ü, ckert-Reed C, Sucipto H, Shu W, Gl, ä, ser L, Patschkowski T, Struck B, Kalinowski J, Luzhetskyy A, Wittmann C, Systems biology of industrial oxytetracycline production in Streptomyces rimosus: the secrets of a mutagenized hyperproducer. Microb Cell Fact, 22(1):222(2023) [pubmed]

10.1186/s12934-023-02215-x;

submitter keyword: Streptomyces rimosus, LC-MSMS, RNAseq

Jörn Kalinowski
contact affiliation	CeBiTec, Bielefeld University
contact email	joern@cebitec.uni-bielefeld.de
lab head
Christian Rückert-Reed
contact affiliation	CeBiTec, Bielefeld University
contact email	christian.rueckert@cebitec.uni-bielefeld.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD042182

PRIDE project URI

• PRIDE
  1. PXD042182
     1. Label: PRIDE project
     2. Name: Multi-omics analysis of mutagenized S. rimosus reveals synergistic changes that drive oxytetracycline production towards industrial efficiency and opens a new route to heterologous polyketides