PXD042174

PXD042174 is an original dataset announced via ProteomeXchange.

Title	Higher-order assembly is a regulatory switch that promotes outer kinetochore recruitment
Description	To faithfully segregate chromosomes during vertebrate mitosis, kinetochore-microtubule interactions must be restricted to a single site on each chromosome. Prior work on pair-wise kinetochore protein interactions has been unable to identify the mechanisms that prevent kinetochore formation in regions with a low density of CENP-A nucleosomes. To investigate the impact of higher-order assembly on kinetochore formation, we generated defined oligomers of the inner kinetochore protein CENP-T using two distinct, genetically engineered systems in human cells. Although individual CENP-T molecules interact poorly with other kinetochore proteins, oligomers that mimic the centromeric density of CENP-T trigger the robust formation of functional, cytoplasmic kinetochore-like particles. Both in cells and in vitro, each molecule of oligomerized CENP-T recruits substantially higher levels of outer kinetochore components than monomeric CENP-T molecules. Thus, the density-dependence of CENP-T restricts outer kinetochore recruitment to centromeres, where densely packed CENP-A recruits a high local concentration of CENP-T.
HostingRepository	PRIDE
AnnounceDate	2023-11-09
AnnouncementXML	Submission_2023-11-08_17:31:06.564.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042174
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	GunterSissoko
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-12 05:30:25	ID requested
⏵ 1	2023-11-08 17:31:06	announced

10.6019/PXD042174;

submitter keyword: CENP-T, Kinetochore

IainMcPherson
contact affiliation	MIT Department of Biology, Cambridge, MA, USA; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
contact email	icheese@wi.mit.edu
lab head
GunterSissoko
contact affiliation	Whitehead Institute
contact email	sissokogunter@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042174
     1. Label: PRIDE project
     2. Name: Higher-order assembly is a regulatory switch that promotes outer kinetochore recruitment