PXD042169
PXD042169 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Datasets for FLASHQuant: a software tool for label-free quantification in top-down proteomics |
| Description | Three datasets with different complexity: ProteinMix, SpikeIn, and ProteomeMix. ProteinMix is the least complex dataset consisting of LC-MS runs with a six-protein mixture (Pierce Intact Protein Standard Mix, A35526, Thermo Fisher). The six proteins are namely Protein G, Protein AG, IGF-I LR3, Thioredoxin, Carbonic Anhydrase II, and Exo Klenow, but due to the MS settings, two large proteins (Protein AG and Exo Klenow) were undetected. To generate the SpikeIn dataset, ProteinMix was spiked into an E. coli lysate in different amounts of ProteinMix (relatively diluted 1, 1/2, 1/3, 1/5, 1/7, and 1/10 with 20 ng/microlitre as 1), each of which was subject to an LC-MS run. The ProteomeMix dataset contains varying the proportion of proteins from the Human Caco-2 cell lysate (1/5, 1/2, 1, 2, and 5) with a constant concentration for E. coli lysate. The ProteinMix and SpikeIn datasets contain triplicates, and the ProteomeMix dataset includes replicates. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-05-23 |
| AnnouncementXML | Submission_2023-05-23_12:31:41.683.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jihyung Kim |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Bos taurus; common name: cattle; NCBI TaxID: 9913; scientific name: Streptococcus; NCBI TaxID: 1301; scientific name: Escherichia coli; common name: E. coli; NCBI TaxID: 562; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-05-12 01:56:39 | ID requested | |
| ⏵ 1 | 2023-05-23 12:31:42 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: top-down proteomics, label-free quantification |
Contact List
| Oliver Kohlbacher | |
|---|---|
| contact affiliation | Tuebingen University |
| contact email | oliver.kohlbacher@uni-tuebingen.de |
| lab head | |
| Jihyung Kim | |
| contact affiliation | University of Tuebingen |
| contact email | jihyung.kim@uni-tuebingen.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091923/ |
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