PXD042168

PXD042168 is an original dataset announced via ProteomeXchange.

Title	Epigenetic Impairment of tumor potential of AML-derived extracellular vesicles
Description	The biological active cargo of leukemia-derived extracellular vesicles (EVs) favours a wide variety of cancer-supportive mechanisms, including aberrant proliferative signalling, immune escape and drug resistance. However, whether and in which extent anti-neoplastic drugs affect EV cargo sorting is often underestimated. Vorinostat, also known as SAHA, has shown promising results against leukemia , and reached important clinical goals when combined with other therapeutics. Nevertheless, SAHA-mediated effects on intercellular communication via EVs remains unknown. Here, we found SAHA to significantly affects the overall cargo associated with leukemia-derived EVs. Interestingly, SAHA differently affects the intracellular and vesicular levels of miR194-5p and BCLAF1 ratio, which imbalance was previously shown to regulate cell survival and differentiation in leukemic cells. Moreover, in silico evaluations predicted SAHA to skew the tumoral potential ascribed to leukemic EVs, hypothesis confirmed by functional evaluations. Finally, EVs from SAHA-treated leukemic cells may condition the sensitivity of surrounding cancer cells to other antineoplastic agents. Altogether we showed that SAHA leads leukemic cells towards apoptosis while reverting the oncologic significance ascribed to leukemia-derived EVs, eventually affecting the efficacy, and therefore selection, of other therapeutics to use for combinatorial purposes.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:29:47.283.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Concetta Cufaro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-12 01:49:38	ID requested
⏵ 1	2025-05-06 09:29:48	announced

10.1186/s12967-025-06361-1;

Massaro C, Sgueglia G, Muro A, Pieragostino D, Lanuti P, Cufaro MC, Giorgio C, D'Agostino E, Torre LD, Baglio SR, Pirozzi M, De Simone M, Altucci L, Dell'Aversana C, Vorinostat impairs the cancer-driving potential of leukemia-secreted extracellular vesicles. J Transl Med, 23(1):421(2025) [pubmed]

submitter keyword: vesicle, leukemia, tumorigenicity,Epigenetic, SAHA

Damiana Pieragostino
contact affiliation	Department of Innovative Technologies in Medicine & Dentistry, University of "G. d'Annunzio" of Chieti-Pescara, Chieti, Italy
contact email	damiana.pieragostino@unich.it
lab head
Maria Concetta Cufaro
contact affiliation	University "G. d'Annunzio" of Chieti
contact email	maria.cufaro@unich.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD042168

PRIDE project URI

• PRIDE
  1. PXD042168
     1. Label: PRIDE project
     2. Name: Epigenetic Impairment of tumor potential of AML-derived extracellular vesicles