PXD042148 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A viral pan-end RNA element and host complex define a SARS-CoV-2 regulon |
| Description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, generates multiple protein-coding, subgenomic RNAs (sgRNAs) from a longer genomic RNA, all bearing identical termini with poorly understood roles in regulating viral gene expression. Insulin and interferon-gamma, two host-derived, stress-related agents, and virus spike protein, induce binding of glutamyl-prolyl-tRNA synthetase (EPRS1), within an unconventional, tetra-aminoacyl-tRNA synthetase complex, to the sgRNA 3'end thereby enhancing sgRNA expression. We identify an EPRS1-binding sarbecoviral pan-end activating RNA (SPEAR) element in the 3'end of viral RNAs driving agonist-induction. Translation of another co-terminal 3'end feature, ORF10, is necessary for SPEAR-mediated induction, independent of Orf10 protein expression. The SPEAR element enhances viral programmed ribosomal frameshifting, thereby expanding its functionality. By co-opting noncanonical activities of a family of essential host proteins, the virus establishes a post-transcriptional regulon stimulating global viral RNA translation. A SPEAR-targeting strategy markedly reduces SARS-CoV-2 titer and kinetics, suggesting a pan-sarbecoviral therapeutic modality. |
| HostingRepository | PRIDE |
| AnnounceDate | 2023-11-14 |
| AnnouncementXML | Submission_2023-11-14_09:01:04.631.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD042148 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ling Li |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-05-11 07:14:41 | ID requested | |
| 1 | 2023-06-12 09:39:14 | announced | |
| ⏵ 2 | 2023-11-14 09:01:05 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: SARS-CoV-2 |
| COVID-19 |
| translational control |
| RNA element |
| RNA-binding protein |
| EPRS1 |
| aminoacyl-tRNA synthetase |
| multi-tRNA synthetase complex |
| post-transcriptional regulon |
| 3’ untranslated region |
| obesity |
| insulin |
| interferon-gamma |
| spike protein |
| subgenomic RNA |
| programmed ribosomal frameshifting |
| SPEAR element |
| ORF10 |
| 3’end |
| SARS-CoV-2 replicon |
| PPMO |
Contact List
| Paul L Fox |
|---|
| contact affiliation | Department of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA |
| contact email | foxp@ccf.org |
| lab head | |
| Ling Li |
|---|
| contact affiliation | Cleveland Clinic |
| contact email | lil5@ccf.org |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD042148 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042148
- Label: PRIDE project
- Name: A viral pan-end RNA element and host complex define a SARS-CoV-2 regulon
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