PXD042142

PXD042142 is an original dataset announced via ProteomeXchange.

Title	Amplified fluorogenic immunoassay for early diagnosis and monitoring of Alzheimer’s disease from tear fluid
Description	Accurate diagnosis of Alzheimer’s disease (AD) in its earliest stage can prevent the disease and delay the onset of symptoms by maintaining a healthy lifestyle and controlling modifiable risk factors. Therefore, more sensitive, relatively inexpensive, non-invasive, and simple screening tools are required for the early diagnosis and progression monitoring of AD. Here, we designed a self assembled nanoparticle-mediated amplified fluorogenic immunoassay (SNAFIA) consisting of magnetic and fluorophore-loaded polymeric nanoparticles. Multiple simultaneous captures and magnetic separation of biomarkers and target-specific fluorophore emission induced a one-tomulti signal, resulting in a low detection limit (236 aM), good reliability (R2 = 0.991), and wide analytical range (0.320–1000 fM) for APOE and CAP1 biomarkers in human serum and tear fluid. More importantly, SNAFIA successfully differentiated AD patients from healthy controls with 90% sensitivity and 100% specificity in less than 1 h in 39 clinical tear samples. As an easily accessible diagnostic tool with fast and accurate results, SNAFIA can help diagnose AD early to aid in long-term care planning, increase the efficiency of clinical trials, and dramatically accelerate therapeutic development.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_10:26:18.946.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chae Eun Moon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-11 04:35:49	ID requested
⏵ 1	2024-01-26 10:26:20	announced

Lee S, Kim E, Moon CE, Park C, Lim JW, Baek M, Shin MK, Ki J, Cho H, Ji YW, Haam S, Amplified fluorogenic immunoassay for early diagnosis and monitoring of Alzheimer's disease from tear fluid. Nat Commun, 14(1):8153(2023) [pubmed]

10.1038/s41467-023-43995-5;

submitter keyword: Alzheimer’s disease, immunoassay, magnetic nanoparticles, polymeric nanoprobes, tear fluid

Yong Woo Ji
contact affiliation	SeRLab (Severance Eye Research Laboratory), Department of Ophthalmology, Yonsei University College of Medicine, Yongin Severance Hospital
contact email	lusita30@yuhs.ac
lab head
Chae Eun Moon
contact affiliation	Yonsei University College of Medicine
contact email	cemoon@yuhs.ac
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD042142

PRIDE project URI

• PRIDE
  1. PXD042142
     1. Label: PRIDE project
     2. Name: Amplified fluorogenic immunoassay for early diagnosis and monitoring of Alzheimer’s disease from tear fluid