PXD042137

PXD042137 is an original dataset announced via ProteomeXchange.

Title	N-glycoproteomics and SESTAR++ analysis based on SeMOE
Description	The isotopic envelope pattern of selenium is guaranteed by its six stable isotopes with distinctive distribution (74Se, 0.89%; 76Se, 9.37%; 77Se, 7.63%; 78Se, 23.77%; 80Se, 49.61%; 82Se, 8.73%). We postulated that the effective incorporation of selenosugar in glycoproteins would achieve direct isotopic pattern prediction for glycopeptides, without the need for secondary tagging procedures. Consequently, we performed comparative studies using a previously reported computational algorithm, the updated version of selenium-encoded isotopic signature targeted profiling (SESTAR++)31, 32, and a glycan-first glycopeptide search engine, pGlyco3 to comprehensively analyze the intact N-glycopeptides in cells treated with SeMOE probes.
HostingRepository	PRIDE
AnnounceDate	2023-11-20
AnnouncementXML	Submission_2023-11-20_00:06:11.543.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiao Tian
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-11 01:02:00	ID requested
⏵ 1	2023-11-20 00:06:11	announced

Dataset with its publication pending

submitter keyword: Human, N-glycoproteomics, HeLa cell, SESTAR, SeMOE

Ran Xie
contact affiliation	State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing, China
contact email	ranxie@nju.edu.cn
lab head
Xiao Tian
contact affiliation	Nanjing University
contact email	tianxiao150200@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD042137

PRIDE project URI

• PRIDE
  1. PXD042137
     1. Label: PRIDE project
     2. Name: N-glycoproteomics and SESTAR++ analysis based on SeMOE