PXD042129

PXD042129 is an original dataset announced via ProteomeXchange.

Title	Target Identification of Lagunamide A
Description	Lagunamide A is a biologically active natural product with a yet unidentified molecular mode of action. Cellular studies revealed that lagunamide A is a potent inhibitor of cancer cell proliferation, promotes apoptosis and causes mitochondrial dysfunction. To decipher the cellular mechanism responsible for these effects, we utilized chemical proteomics and identified EYA3 as a target of lagunamide A. EYA3 was functionally validated via knockout and its role for the anti-proliferative and pro-apoptotic effects of lagunamide A was confirmed. As EYA3 turned out to be important for DNA damage repair, we showed that lagunamide A sensitized tumor cells to treatment with the drug doxorubicin highlighting a putative therapeutic strategy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:44:13.473.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dietrich Mostert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-10 12:40:59	ID requested
1	2024-06-09 06:57:31	announced
⏵ 2	2024-10-22 06:44:14	announced	2024-10-22: Updated project metadata.

Hu Y, Mostert D, Orgler C, Andler O, Zischka H, Kazmaier U, Vollmar AM, Braig S, Sieber SA, Zahler S, Thermal Proteome Profiling Reveals Insight to Antiproliferative and Pro-Apoptotic Effects of Lagunamide A in the Modulation of DNA Damage Repair. Chembiochem, 25(13):e202400024(2024) [pubmed]

10.1002/cbic.202400024;

submitter keyword: Natural product, TMT-10plex, Lagunamide A, Thermal Proteome Profiling,Target ID

Stephan Sieber
contact affiliation	Center for Functional Protein Assemblies (CPA), Department of Chemistry, Chair of organic Chemistry II, Technical University of Munich
contact email	stephan.sieber@tum.de
lab head
Dietrich Mostert
contact affiliation	Center for Functional Protein Assemblies (CPA), Department of Chemistry, Chair of Organic Chemistry II, Technical University of Munich, 85748 Garching, Germany.
contact email	dietrich.mostert@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD042129

PRIDE project URI

• PRIDE
  1. PXD042129
     1. Label: PRIDE project
     2. Name: Target Identification of Lagunamide A