PXD042128

PXD042128 is an original dataset announced via ProteomeXchange.

Title	Mouse Kidney MS - Standas diet WT/ High fat diet WT/ Standard diet ANT2 KO/ High fat diet ANT2 KO
Description	Impairments in ATP production and transport in renal proximal tubule cells (RPTCs) result in obesity-induced chronic kidney disease (CKD), manifested by kidney dysfunction, inflammation, adiposity, and fibrosis. Here we assessed the role of adenine nucleotide transporter 2 (ANT2), the main regulator of cellular ATP content in RPTCs, in the development of obesity-induced CKD and its metabolic abnormalities. Obese RPTC-ANT2-/- mice exhibited normal renal morphology, function, and lack of kidney adiposity and fibrosis as well as an improvement in whole-body energy metabolism, manifested by normal glucose and lipid homeostasis and reduced hepatic steatosis. ANT2-depleted RPTCs rewired their primary metabolic program from oxidizing fatty acids as a primary energy source toward aerobic glycolysis, mediated via the testis-selective ANT4. We propose that RPTC-ANT2 plays an important role in the development of obesity-induced CKD, and that its nullification triggers mitochondrial protection, RPTC cellular survival, kidney preservation, and improvements in systemic metabolism.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:41:32.563.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Permyakova
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-10 11:22:52	ID requested
⏵ 1	2025-05-06 09:41:33	announced

10.1681/asn.0000000000000294;

Permyakova A, Hamad S, Hinden L, Baraghithy S, Kogot-Levin A, Yosef O, Shalev O, Tripathi MK, Amal H, Basu A, Arif M, Cinar R, Kunos G, Berger M, Leibowitz G, Tam J, Renal Mitochondrial ATP Transporter Ablation Ameliorates Obesity-Induced CKD. J Am Soc Nephrol, 35(3):281-298(2024) [pubmed]

submitter keyword: Mouse, Kidney, MS

Joseph Tam
contact affiliation	Obesity and Metabolism Laboratory, The Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Israel.
contact email	yossi.tam@mail.huji.ac.il
lab head
Anna Permyakova
contact affiliation	Hebrew University of Jerusalem
contact email	anna.permyakova@mail.huji.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD042128

PRIDE project URI

• PRIDE
  1. PXD042128
     1. Label: PRIDE project
     2. Name: Mouse Kidney MS - Standas diet WT/ High fat diet WT/ Standard diet ANT2 KO/ High fat diet ANT2 KO