PXD042118

PXD042118 is an original dataset announced via ProteomeXchange.

Title	Bacteroidota assemble a unique type VI secretion system membrane complex
Description	Microbes have evolved elaborate mechanisms to cope with competitors, including the type VIsecretion system (T6SS) of Gram-negative bacteria. The T6SS inhibits target cells through contact-dependent translocation of toxic effector proteins across two cellular membranes via an inverted phage-like apparatus. Proteobacteria accomplish this feat by passage of theT6SS needle through a megadalton-size membrane complex (MC), which is essential for T6SS function. Remarkably, although the phylum Bacteroidota encodes a T6SS, it lacks homologs of the MC. We identified five novel genes, essential for T6SS function, that encode a candidate unique Bacteroidota T6SSMC. We purified the T6SSiii MC and revealed its dimensions using electron microscopy. We identified an intricate protein protein interaction network underlying the assembly of the MC, the stoichiometry of the five TssNQOPR components., tTheir predicted structures were validated using crosslinking mass-spectrometry and we assessed the structural homology with known proteins. Importantly, we determine the connection between the T6SSiii MC and the otherwise conserved baseplate involving the hub protein Tss.Finally, phylogenomic analysis of the distribution of T6SSiii MC genes across the phylum Bacteroidota highlights patterns of conservation including the invariant
HostingRepository	PRIDE
AnnounceDate	2024-01-03
AnnouncementXML	Submission_2024-01-03_12:17:52.262.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stengel
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-10 07:01:02	ID requested
⏵ 1	2024-01-03 12:17:52	announced

Dataset with its publication pending

submitter keyword: Type VI secretion,Bacteria, membrane protein

Florian Stengel
contact affiliation	Department of Biology, AG Stengel, University of Konstanz,Germany
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliation	Universität Konstanz
contact email	florian.stengel@uni-konstanz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD042118

PRIDE project URI

• PRIDE
  1. PXD042118
     1. Label: PRIDE project
     2. Name: Bacteroidota assemble a unique type VI secretion system membrane complex