PXD042033

PXD042033 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based quantitative proteomic profiling of Natranaerobius thermophilus in response to salinity stress
Description	Natranaerobius thermophilus is a unique extremophile, which exhibits high adaptability and preferability to the combined extreme conditions of high salt concentration, alkaline pH and elevated temperature. At present, the global regulation of genes and proteins in the salinity adaptation strategies of N. thermophilus remains unclear. In this study, we employed the isobaric tags for relative and absolute quantification (iTRAQ) method to investigate the protein abundances of N. thermophilus in response to different Na+ concentrations (2.5 M, 3.1 M, 3.7 M, 4.3 M) at high alkali (pH55°C9.5) and high temperature (53℃). In addition, the transcriptional expression level of some differentially expressed proteins, the expression pattern clustering, and the protein-protein interaction network were analyzed. A total of 1490 proteins were identified, and 659 proteins displayed significant expression under 3.1M (HS3.1 group), 3.7M (HS3.7 group), and 4.3M (HS4.3 group) Na+ concentrations compared with the 2.5M Na+ concentrations (CK2.5 group). Of them, 59 showed simultaneously higher expression and 91 showed simultaneously lower expression. Both GO, COG and KEGG enrichment analysis revealed that up-regulate expressed genes were enriched in membrane transporters, amino acid transport and metabolism, catalytic activity, the TCA cycle, detoxification and stress response and metabolic pathways. Among them, 59 upregulated simultaneously proteins were analyzed and confirmed at the gene expression level by Droplet Digital PCR (ddPCR). Based on our results, we propose that under extremely high salinity pressure，cell membrane high activity, increased biosynthesis and transportation of osmotic protectants, and increased energy production and conversion contribute to the Salt stress tolerance of N. thermophilus. The aim of this study was to provide new insight into the response of N. thermophilus to high-salt at alkaline pH and elevated temperature and identify mechanisms responsible for this adaptation.
HostingRepository	iProX
AnnounceDate	2023-05-06
AnnouncementXML	Submission_2024-01-03_20:01:33.714.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qinghua Xing
SpeciesList	scientific name: Natranaerobius thermophilus JW/NM-WN-LF; NCBI TaxID: 457570;
ModificationList	iTRAQ4plex reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-05 21:48:56	ID requested
⏵ 1	2024-01-03 20:01:34	announced

Dataset with its publication pending

submitter keyword: Natranaerobius thermophilus, salinity stress, iTRAQ, proteomic

Baisuo Zhao
contact affiliation	Graduate School of Chinese Academy of Agriculture
contact email	bszhao@163.com
lab head
Qinghua Xing
contact affiliation	Graduate School of Chinese Academy of Agriculture
contact email	xingqinghua7777@163.com
dataset submitter

iProX dataset URI